{
"@context": "/terms/",
"@id": "/construct-library-sets/IGVFDS9629YZLO/",
"@type": [
"ConstructLibrarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS9629YZLO",
"aliases": [
"gary-hon:CM_Pilot_02_sgRNA_list"
],
"applied_to_samples": [
{
"@id": "/in-vitro-systems/IGVFSM2709AONE/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2709AONE",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_PB"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM4700PDTH/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4700PDTH",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_Lenti"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM8305VDSK/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8305VDSK",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_Lenti"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM1649MDAH/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1649MDAH",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_PB"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM9223FAYO/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM9223FAYO",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_Lenti_LW269"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW269) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM6628JOOL/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6628JOOL",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_Lenti_LW270"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW270) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM5657YKPN/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5657YKPN",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_Lenti_LW271"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW271) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM1608DJBB/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1608DJBB",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_Lenti_LW272"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW272) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM1063UQVF/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1063UQVF",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_PB_LW273"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW273) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM5849QIEI/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5849QIEI",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_PB_LW274"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW274) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM9532RAXS/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM9532RAXS",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_PB_LW275"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW275) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM6716LOHW/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6716LOHW",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_PB_LW276"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"status": "released",
"term_name": "H9"
}
],
"status": "preview",
"summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW276) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM0248SELV/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0248SELV",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_Lenti_LW262"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW262) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM4930FDUF/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4930FDUF",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_Lenti_LW266"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW266) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM8137TZJW/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8137TZJW",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_PB_LW234"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW264) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM7215JDPM/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7215JDPM",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_Lenti_LW265"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW265) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM1972EKCJ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1972EKCJ",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_Lenti_LW261"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW261) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM0333RWYR/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0333RWYR",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_Lenti_LW263"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW263) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM0981UTUA/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0981UTUA",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_Lenti_LW264"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW264) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM5628VVVG/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5628VVVG",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_Lenti_LW267"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW267) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM2215YBLM/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2215YBLM",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_Lenti_LW268"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW268) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM7740VKXC/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7740VKXC",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_PB_LW231"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW261) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM4953FLXN/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4953FLXN",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_PB_LW232"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW262) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM9371INZC/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM9371INZC",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_PB_LW233"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW263) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM2930BIPX/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2930BIPX",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_PB_LW235"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW265) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM5652SBGF/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5652SBGF",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_PB_LW236"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW266) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM2329UXLZ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2329UXLZ",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_PB_LW237"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW267) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
},
{
"@id": "/in-vitro-systems/IGVFSM9326DRHX/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM9326DRHX",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot1_PB_LW238"
],
"classifications": [
"differentiated cell specimen"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"nucleic_acid_delivery": "transfection",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW268) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
}
}
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Preview construct library set [IGVFDS9629YZLO](/construct-library-sets/IGVFDS9629YZLO/) has in progress subobject tabular file [IGVFFI4543YHDW](/tabular-files/IGVFFI4543YHDW/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS9629YZLO/"
}
],
"NOT_COMPLIANT": [
{
"category": "missing plasmid map",
"detail": "Construct library set [IGVFDS9629YZLO](/construct-library-sets/IGVFDS9629YZLO/) does not have a plasmid map attached in `documents`. Construct library sets are expected to be associated with a plasmid map document.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_construct_library_set_plasmid_map",
"path": "/construct-library-sets/IGVFDS9629YZLO/"
}
]
},
"award": {
"@id": "/awards/HG011996/",
"component": "functional characterization"
},
"control_for": [],
"creation_timestamp": "2024-02-15T17:26:04.417309+00:00",
"file_set_type": "guide library",
"files": [],
"guide_type": "sgRNA",
"input_for": [],
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI4543YHDW/",
"accession": "IGVFFI4543YHDW",
"aliases": [
"gary-hon:CM_Pilot02_sgRNA_file"
],
"content_type": "guide RNA sequences",
"file_format": "csv",
"status": "in progress",
"upload_status": "invalidated"
}
],
"lab": {
"@id": "/labs/gary-hon/",
"title": "Gary Hon, UT"
},
"large_scale_loci_list": {
"@id": "/tabular-files/IGVFFI4543YHDW/",
"accession": "IGVFFI4543YHDW",
"aliases": [
"gary-hon:CM_Pilot02_sgRNA_file"
],
"status": "in progress"
},
"schema_version": "10",
"scope": "loci",
"selection_criteria": [
"accessible genome regions"
],
"status": "preview",
"submitted_by": {
"@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
"title": "Mpathi Nzima"
},
"submitter_comment": "sgRNA reverse compliment sequences used for FBA sgRNA mapping. Loci list will be updated, but currently all the loci can be extracted from the sgRNA file. Reach out to the the Hon Lab for any further questions",
"summary": "guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"uuid": "f832045a-e33f-4f11-a8a3-9fd944c6e752"
}