{
"@context": "/terms/",
"@id": "/construct-library-sets/IGVFDS8345ZMZW/",
"@type": [
"ConstructLibrarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS8345ZMZW",
"aliases": [
"charles-gersbach:cd8-tcell-cd2-grna-construct-lib-set"
],
"applied_to_samples": [
{
"@id": "/primary-cells/IGVFSM1730FNFX/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1730FNFX",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispri-cd2-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4080HFHD/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM3002NRDK/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3002NRDK",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispri-cd2-rep3"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1423VLXL/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM4694MBIW/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4694MBIW",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispri-cd2-low-bin-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4080HFHD/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM7030KVXG/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7030KVXG",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispri-cd2-high-bin-rep3"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1423VLXL/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM6350DJQS/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6350DJQS",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispri-cd2-low-bin-rep3"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1423VLXL/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM7658WDNM/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7658WDNM",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispri-cd2-high-bin-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4080HFHD/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
}
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released construct library set [IGVFDS8345ZMZW](/construct-library-sets/IGVFDS8345ZMZW/) has in progress subobject tabular file [IGVFFI9251ZEHU](/tabular-files/IGVFFI9251ZEHU/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS8345ZMZW/"
}
],
"WARNING": [
{
"category": "missing files",
"detail": "Construct library set [IGVFDS8345ZMZW](/construct-library-sets/IGVFDS8345ZMZW/) has no `files`. File sets are expected to have files.",
"level": 40,
"level_name": "WARNING",
"name": "audit_no_files",
"path": "/construct-library-sets/IGVFDS8345ZMZW/"
}
]
},
"average_guide_coverage": 141,
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"control_for": [],
"creation_timestamp": "2023-11-08T18:20:37.850914+00:00",
"documents": [
"/documents/27ec67ed-40bb-4090-93db-a7b2d9fc1861/"
],
"file_set_type": "guide library",
"files": [],
"guide_type": "sgRNA",
"input_for": [],
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI9251ZEHU/",
"accession": "IGVFFI9251ZEHU",
"aliases": [
"charles-gersbach:cd8-tcell-cd2-grnas-metadata"
],
"content_type": "guide RNA sequences",
"file_format": "tsv",
"upload_status": "invalidated"
}
],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"release_timestamp": "2024-11-18T21:09:03.307109+00:00",
"schema_version": "10",
"scope": "genes",
"selection_criteria": [
"transcription start sites"
],
"small_scale_gene_list": [
{
"@id": "/genes/ENSG00000116824/",
"geneid": "ENSG00000116824",
"name": "CD2 molecule",
"symbol": "CD2",
"synonyms": [
"SRBC",
"LFA-3 receptor",
"T11",
"rosette receptor",
"erythrocyte receptor",
"LFA-2",
"CD2 antigen (p50), sheep red blood cell receptor",
"T-cell surface antigen T11/Leu-5",
"lymphocyte-function antigen-2",
"T-cell surface antigen CD2"
]
}
],
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"summary": "Guide (sgRNA) library targeting transcription start sites in CD2",
"tiling_modality": "peak tiling",
"uuid": "dd606d05-1203-4430-b4af-fc6ecfe73142"
}