{
"@context": "/terms/",
"@id": "/construct-library-sets/IGVFDS8183QSJO/",
"@type": [
"ConstructLibrarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS8183QSJO",
"aliases": [
"charles-gersbach:jurkat-il2ra-grna-construct-lib-set"
],
"applied_to_samples": [
{
"@id": "/in-vitro-systems/IGVFSM6373RONE/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6373RONE",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64-il2ra-rep2"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2154QIFS/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM6721RTTV/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6721RTTV",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-rep2"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2560LMUL/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM3606VJWU/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3606VJWU",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64-il2ra-low-bin-rep3"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1648DRBT/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM1838CFFU/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1838CFFU",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-low-bin-rep1"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO9862HFWL/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM2737ATVK/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2737ATVK",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64-il2ra-high-bin-rep2"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2154QIFS/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM3244YHOI/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3244YHOI",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-high-bin-rep3"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4048MCTZ/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM8744PJOL/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8744PJOL",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64-il2ra-rep3"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1648DRBT/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM1480DJBL/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1480DJBL",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64-il2ra-low-bin-rep1"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0512VHUM/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM4817IZZH/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4817IZZH",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-high-bin-rep1"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO9862HFWL/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM6606RDWT/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6606RDWT",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-rep3"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4048MCTZ/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM3148FRBY/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3148FRBY",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64-il2ra-high-bin-rep1"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0512VHUM/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM7748IVLZ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7748IVLZ",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64-il2ra-high-bin-rep3"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1648DRBT/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM6490XCFZ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6490XCFZ",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64-il2ra-rep1"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0512VHUM/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM6178KGGZ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6178KGGZ",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-rep1"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO9862HFWL/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM4401YRWQ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4401YRWQ",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64-il2ra-low-bin-rep2"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2154QIFS/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM6964ODEE/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6964ODEE",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-high-bin-rep2"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2560LMUL/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM2429HKWG/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2429HKWG",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-low-bin-rep2"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2560LMUL/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/in-vitro-systems/IGVFSM5112IFQB/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5112IFQB",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-low-bin-rep3"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4048MCTZ/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)"
}
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released construct library set [IGVFDS8183QSJO](/construct-library-sets/IGVFDS8183QSJO/) has in progress subobject tabular file [IGVFFI3477BKFD](/tabular-files/IGVFFI3477BKFD/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS8183QSJO/"
}
],
"WARNING": [
{
"category": "missing files",
"detail": "Construct library set [IGVFDS8183QSJO](/construct-library-sets/IGVFDS8183QSJO/) has no `files`. File sets are expected to have files.",
"level": 40,
"level_name": "WARNING",
"name": "audit_no_files",
"path": "/construct-library-sets/IGVFDS8183QSJO/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"control_for": [],
"creation_timestamp": "2023-11-08T18:25:50.552331+00:00",
"documents": [
"/documents/59f49737-feb9-43b5-99e3-0828577c240a/"
],
"file_set_type": "guide library",
"files": [],
"guide_type": "sgRNA",
"input_for": [],
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI3477BKFD/",
"accession": "IGVFFI3477BKFD",
"aliases": [
"charles-gersbach:jurkat-il2ra-grnas-metadata"
],
"content_type": "guide RNA sequences",
"file_format": "tsv",
"upload_status": "invalidated"
}
],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"release_timestamp": "2024-11-18T21:08:54.764033+00:00",
"schema_version": "10",
"scope": "genes",
"selection_criteria": [
"transcription start sites"
],
"small_scale_gene_list": [
{
"@id": "/genes/ENSG00000134460/",
"geneid": "ENSG00000134460",
"name": "interleukin 2 receptor subunit alpha",
"symbol": "IL2RA",
"synonyms": [
"interleukin-2 receptor subunit alpha",
"IL2R",
"TAC antigen",
"IMD41",
"IL-2R subunit alpha",
"CD25",
"IL-2 receptor subunit alpha",
"IDDM10",
"TCGFR",
"p55",
"interleukin 2 receptor, alpha"
]
}
],
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"summary": "Guide (sgRNA) library targeting transcription start sites in IL2RA",
"tiling_modality": "peak tiling",
"uuid": "7e775f53-b647-47a5-91f3-02250c3a3628"
}