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IGVFDS7621RRLM

released
File Set Type
guide library
Summary
guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Description
gRNAs were designed to target DNase peak in TeloHAEC in chr15:80,975,273-81,134,905 to determine regulatory elements of TLNRD1
Aliases
jesse-engreitz:construct_library_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH
Submitter Comment
Guide (sgRNA) library targeting DNase peaks around rs1879454 in TeloHAEC

Guide Library Details

Library Construct Scope
loci
Selection Criteria
candidate cis-regulatory elements
Small Scale Loci List
GRCh38 chr15 80975273-81134905
Guide Type
sgRNA
Average Guide Coverage
139285

Integrated Content Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bed
guide RNA sequences
GRCh38 guide RNA sequences
Jesse Engreitz, Stanford
3.9 KB
validated

Applied to Samples

21 items
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 26-50% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Documents

2 items
Page
Preview
sgOpti-blast gRNA plasmid map
plasmid map
sgOpti-blast gRNA plasmid sequence
plasmid map

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford