{
"@context": "/terms/",
"@id": "/construct-library-sets/IGVFDS7189YPQQ/",
"@type": [
"ConstructLibrarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS7189YPQQ",
"aliases": [
"charles-gersbach:cd8-tcell-ko-znf217-grna-construct-lib-set"
],
"applied_to_samples": [
{
"@id": "/primary-cells/IGVFSM5367RVVU/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5367RVVU",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-cas9-ko-znf217-rep3"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4048LCOU/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM1311HWOF/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1311HWOF",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-cas9-ko-znf217-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO7589UJKG/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM5949AWIH/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5949AWIH",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-cas9-ko-znf217-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8565PQXZ/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
}
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released construct library set [IGVFDS7189YPQQ](/construct-library-sets/IGVFDS7189YPQQ/) has in progress subobject tabular file [IGVFFI9240JENT](/tabular-files/IGVFFI9240JENT/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS7189YPQQ/"
}
],
"WARNING": [
{
"category": "missing files",
"detail": "Construct library set [IGVFDS7189YPQQ](/construct-library-sets/IGVFDS7189YPQQ/) has no `files`. File sets are expected to have files.",
"level": 40,
"level_name": "WARNING",
"name": "audit_no_files",
"path": "/construct-library-sets/IGVFDS7189YPQQ/"
}
]
},
"average_guide_coverage": 1,
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"control_for": [],
"creation_timestamp": "2023-11-08T18:25:48.476942+00:00",
"documents": [
"/documents/2850de29-469d-445e-b303-9753b6f878ea/"
],
"file_set_type": "guide library",
"files": [],
"guide_type": "sgRNA",
"input_for": [],
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI9240JENT/",
"accession": "IGVFFI9240JENT",
"aliases": [
"charles-gersbach:cd8-tcell-ko-znf217-grnas-metadata"
],
"content_type": "guide RNA sequences",
"file_format": "tsv",
"upload_status": "invalidated"
}
],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"release_timestamp": "2024-10-25T04:18:39.352797+00:00",
"schema_version": "10",
"scope": "genes",
"selection_criteria": [
"transcription start sites"
],
"small_scale_gene_list": [
{
"@id": "/genes/ENSG00000171940/",
"geneid": "ENSG00000171940",
"name": "zinc finger protein 217",
"symbol": "ZNF217",
"synonyms": [
"zinc finger protein 217",
"ZABC1"
]
}
],
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"summary": "Guide (sgRNA) library targeting transcription start sites in ZNF217",
"tiling_modality": "sparse peaks",
"uuid": "e47ad8e1-8076-425f-b786-aef89a0e9cc6"
}