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IGVFDS6102AFVS

released
File Set Type
guide library
Summary
guide (sgRNA) library targeting transcription start sites in many genes
Description
Perturb-seq CRISPRa library: gRNA library of all targeting hits (padj<.01) from the TFome screen with non-targeting gRNAs
Aliases
charles-gersbach:hPA-ConstructLibrarySet-perturb-seq-library
Attribution
Charles Gersbach, Duke

Guide Library Details

Library Construct Scope
genes
Selection Criteria
transcription start sites
Large Scale Gene List
IGVFFI4279ZUTM
Guide Type
sgRNA
Tiling Modality
sparse peaks

Integrated Content Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
guide RNA sequences
guide RNA sequences
Charles Gersbach, Duke
4.1 KB
validated

Applied to Samples

3 items
Sample Terms
Summary
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library

Documents

1 item
Page
Description
Type
Download
Standardized File Format
Preview
Plasmid for CRISPRa with PuroR
plasmid map