{
"@context": "/terms/",
"@id": "/construct-library-sets/IGVFDS3667PLQB/",
"@type": [
"ConstructLibrarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS3667PLQB",
"aliases": [
"charles-gersbach:tfome-grna-construct-lib-set"
],
"applied_to_samples": [
{
"@id": "/primary-cells/IGVFSM8084QKPW/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8084QKPW",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-high-bin-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8306NDTY/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM9974ALBF/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM9974ALBF",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4646JDEN/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM7887TFLH/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7887TFLH",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-high-bin-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2763RVOY/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM4936HKEW/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4936HKEW",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-low-bin-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4646JDEN/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM7158ADKU/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7158ADKU",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-low-bin-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2763RVOY/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM0466RHGQ/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0466RHGQ",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-high-bin-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4646JDEN/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM7226CVDS/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7226CVDS",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-high-bin-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO6692QWLN/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM3910UHIY/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3910UHIY",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO6692QWLN/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM2824DRGN/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2824DRGN",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8306NDTY/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM2082DWDD/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2082DWDD",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2763RVOY/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM2624KTRK/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2624KTRK",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-low-bin-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO6692QWLN/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM3895SURE/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3895SURE",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-low-bin-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8306NDTY/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
}
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released construct library set [IGVFDS3667PLQB](/construct-library-sets/IGVFDS3667PLQB/) has in progress subobject reference file [IGVFFI6537JARB](/reference-files/IGVFFI6537JARB/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS3667PLQB/"
},
{
"category": "mismatched status",
"detail": "Released construct library set [IGVFDS3667PLQB](/construct-library-sets/IGVFDS3667PLQB/) has in progress subobject tabular file [IGVFFI9735DZKB](/tabular-files/IGVFFI9735DZKB/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS3667PLQB/"
}
],
"WARNING": [
{
"category": "missing files",
"detail": "Construct library set [IGVFDS3667PLQB](/construct-library-sets/IGVFDS3667PLQB/) has no `files`. File sets are expected to have files.",
"level": 40,
"level_name": "WARNING",
"name": "audit_no_files",
"path": "/construct-library-sets/IGVFDS3667PLQB/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"control_for": [],
"creation_timestamp": "2023-11-08T18:25:52.688049+00:00",
"documents": [
"/documents/214fde61-7d80-472a-b9c2-4baab326c9de/",
"/documents/2850de29-469d-445e-b303-9753b6f878ea/"
],
"file_set_type": "guide library",
"files": [],
"guide_type": "sgRNA",
"input_for": [],
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI9735DZKB/",
"accession": "IGVFFI9735DZKB",
"aliases": [
"charles-gersbach:tfome-grnas-metadata"
],
"content_type": "guide RNA sequences",
"file_format": "tsv",
"upload_status": "invalidated"
}
],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"large_scale_gene_list": {
"@id": "/reference-files/IGVFFI6537JARB/",
"accession": "IGVFFI6537JARB"
},
"release_timestamp": "2024-11-18T21:09:01.381380+00:00",
"schema_version": "10",
"scope": "genes",
"selection_criteria": [
"transcription start sites"
],
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"summary": "Guide (sgRNA) library targeting transcription start sites in many genes",
"tiling_modality": "sparse peaks",
"uuid": "5e2e4639-cc3b-46ad-bbc2-716cea214506"
}