{
"@context": "/terms/",
"@id": "/construct-library-sets/IGVFDS1868WXHB/",
"@type": [
"ConstructLibrarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS1868WXHB",
"aliases": [
"charles-gersbach:cd8-tcell-overexpression-batf3-construct-library"
],
"applied_to_samples": [
{
"@id": "/primary-cells/IGVFSM2624PYEY/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2624PYEY",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-batf3-rep4"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO7613AWPB/",
"status": "in progress",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "in progress",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/725c21b1-d202-4a6c-89a4-e4fc05a001e8/",
"status": "in progress",
"summary": "Treatment of 300 percent Antibody Coated Bead",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM0846TBSG/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0846TBSG",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-batf3-rep5"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0626AJQM/",
"status": "in progress",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "in progress",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/725c21b1-d202-4a6c-89a4-e4fc05a001e8/",
"status": "in progress",
"summary": "Treatment of 300 percent Antibody Coated Bead",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM0218YCAH/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0218YCAH",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-chronic-activation-batf3-rep3"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8289ZMDD/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead for 14 days, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/451e1aee-b7f5-4a04-9fa5-672390a2506d/",
"status": "released",
"summary": "Treatment of 300 percent Antibody Coated Bead for 14 days",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM0619UUOC/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0619UUOC",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-batf3-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO7858JPMS/",
"status": "in progress",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "in progress",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/725c21b1-d202-4a6c-89a4-e4fc05a001e8/",
"status": "in progress",
"summary": "Treatment of 300 percent Antibody Coated Bead",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM8172ZPYG/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8172ZPYG",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-batf3-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4396YGDT/",
"status": "in progress",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "in progress",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/725c21b1-d202-4a6c-89a4-e4fc05a001e8/",
"status": "in progress",
"summary": "Treatment of 300 percent Antibody Coated Bead",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM0066TACG/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0066TACG",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-acute-activation-batf3-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO9588OERW/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/725c21b1-d202-4a6c-89a4-e4fc05a001e8/",
"status": "in progress",
"summary": "Treatment of 300 percent Antibody Coated Bead",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM7232YAVV/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7232YAVV",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-chronic-activation-batf3-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1522NSLA/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead for 14 days, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/451e1aee-b7f5-4a04-9fa5-672390a2506d/",
"status": "released",
"summary": "Treatment of 300 percent Antibody Coated Bead for 14 days",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM2391CIZT/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2391CIZT",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-batf3-rep3"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0178WWBR/",
"status": "in progress",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "in progress",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/725c21b1-d202-4a6c-89a4-e4fc05a001e8/",
"status": "in progress",
"summary": "Treatment of 300 percent Antibody Coated Bead",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM4716FORO/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4716FORO",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-acute-activation-batf3-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO1522NSLA/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/725c21b1-d202-4a6c-89a4-e4fc05a001e8/",
"status": "in progress",
"summary": "Treatment of 300 percent Antibody Coated Bead",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM0293OVAT/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0293OVAT",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-acute-activation-batf3-rep3"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8289ZMDD/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/725c21b1-d202-4a6c-89a4-e4fc05a001e8/",
"status": "in progress",
"summary": "Treatment of 300 percent Antibody Coated Bead",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM8821YQXI/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8821YQXI",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-chronic-activation-batf3-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO9588OERW/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell treated with 300 percent Antibody Coated Bead for 14 days, transduced (lentivirus) with a expression vector library",
"treatments": [
{
"@id": "/treatments/451e1aee-b7f5-4a04-9fa5-672390a2506d/",
"status": "released",
"summary": "Treatment of 300 percent Antibody Coated Bead for 14 days",
"treatment_term_name": "Antibody Coated Bead"
}
]
},
{
"@id": "/primary-cells/IGVFSM3895SURE/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3895SURE",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-low-bin-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8306NDTY/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM7887TFLH/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7887TFLH",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-high-bin-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2763RVOY/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM7158ADKU/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7158ADKU",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-low-bin-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2763RVOY/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM8084QKPW/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8084QKPW",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-high-bin-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8306NDTY/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM2824DRGN/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2824DRGN",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8306NDTY/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM2082DWDD/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2082DWDD",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO2763RVOY/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)"
}
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing publication",
"detail": "Construct library set [IGVFDS1868WXHB](/construct-library-sets/IGVFDS1868WXHB/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/construct-library-sets/IGVFDS1868WXHB/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"control_file_sets": [
"/construct-library-sets/IGVFDS5584SPIV/"
],
"control_for": [],
"creation_timestamp": "2024-10-28T18:17:50.740157+00:00",
"documents": [
"/documents/d25518aa-6bcb-43ce-a2f0-8d16f513ff0a/"
],
"file_set_type": "expression vector library",
"files": [],
"input_for": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"release_timestamp": "2024-10-29T17:33:31.650435+00:00",
"schema_version": "10",
"scope": "genes",
"selection_criteria": [
"genes"
],
"small_scale_gene_list": [
{
"@id": "/genes/ENSG00000123685/",
"geneid": "ENSG00000123685",
"name": "basic leucine zipper ATF-like transcription factor 3",
"status": "released",
"symbol": "BATF3",
"synonyms": [
"basic leucine zipper transcriptional factor ATF-like 3",
"21-kD small nuclear factor isolated from T cells",
"B-ATF-3",
"Jun dimerization protein 1",
"SNFT",
"basic leucine zipper transcription factor, ATF-like 3",
"21 kDa small nuclear factor isolated from T-cells",
"JUNDM1",
"basic leucine zipper ATF-like transcription factor 3",
"BATF3",
"JDP1",
"Jun dimerization protein p21SNFT"
]
}
],
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"summary": "expression vector library of BATF3",
"uuid": "98457539-65fd-4019-b3b1-9d2077c6b9e8"
}