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IGVFDS0821YMMV

released
File Set Type
guide library
Summary
guide (sgRNA) library targeting transcription start sites in 24 genes
Aliases
charles-gersbach:cd8-tcell-perturb-seq-crispr-grna-construct-lib-set

Guide Library Details

Library Construct Scope
genes
Selection Criteria
transcription start sites
Small Scale Gene List
Guide Type
sgRNA
Tiling Modality
sparse peaks
Average Guide Coverage
1.33

Integrated Content Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
guide RNA sequences
GRCh38 guide RNA sequences
Charles Gersbach, Duke
1.4 KB
validated

Applied to Samples

6 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Documents

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke