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IGVFDS9055BZJI

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin6hrs-FF1

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
482 B
validated

Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

6 items
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR1_Rep1_S115
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR1_Rep2_S170
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR2_Rep1_S121
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR2_Rep2_S176
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR3_Rep1_S127
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR3_Rep2_S182
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR4_Rep1_S133
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR4_Rep2_S188
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep1_S114
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep2_S169
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep1_S120
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep2_S175
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep1_S126
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep2_S181
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep1_S132
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep2_S187
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep1_S116
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep2_S171
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep1_S122
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep2_S177
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep1_S128
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep2_S183
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep1_S134
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep2_S189
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep1_S112
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep2_S167
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep1_S118
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep2_S173
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep1_S124
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep1_S130
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR1_Rep1_S113
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR1_Rep2_S168
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR2_Rep1_S119
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR2_Rep2_S174
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR3_Rep1_S125
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR3_Rep2_S180
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR4_Rep1_S131
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR4_Rep2_S186
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep1_S111
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep2_S166
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep1_S117
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep2_S172
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep1_S123
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep2_S178
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep1_S129
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep2_S184
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford