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IGVFDS8896GBSX

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR1_S5
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR2_S101
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR3_S197
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR4_S293

Sequencing Results

4 items
fastq
reads
1
AGCAATTC:TGCG
9.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AGCAATTC:CATA
9.9 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AGCAATTC:ACGT
8.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AGCAATTC:GTAC
11 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR1_S5
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR2_S101
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR3_S197
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR4_S293
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford