{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS8878JVPZ/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS8878JVPZ",
"aliases": [
"gary-hon:differentited_WTC11_CM_Pilot2_Mini_PB9_HTO_LW437"
],
"audit": {
"ERROR": [
{
"category": "inconsistent sequencing kit",
"detail": "Auxiliary set [IGVFDS8878JVPZ](/auxiliary-sets/IGVFDS8878JVPZ/) has a sequence file [IGVFFI1394EWKL](/sequence-files/IGVFFI1394EWKL/) sequenced on a `sequencing_platform` [EFO_0008637](/platform-terms/EFO_0008637/) that is inconsistent with its `sequencing_kit` NovaSeq X Series 25B Reagent Kit. Sequence files should specify a sequencing kit which is consistent with their sequencing platform. Sequence files in the same sequencing run should also specify the same sequencing kit.",
"level": 60,
"level_name": "ERROR",
"name": "audit_inconsistent_sequencing_kit",
"path": "/auxiliary-sets/IGVFDS8878JVPZ/"
},
{
"category": "inconsistent sequencing kit",
"detail": "Auxiliary set [IGVFDS8878JVPZ](/auxiliary-sets/IGVFDS8878JVPZ/) has a sequence file [IGVFFI7023BMQV](/sequence-files/IGVFFI7023BMQV/) sequenced on a `sequencing_platform` [EFO_0008637](/platform-terms/EFO_0008637/) that is inconsistent with its `sequencing_kit` NovaSeq X Series 25B Reagent Kit. Sequence files should specify a sequencing kit which is consistent with their sequencing platform. Sequence files in the same sequencing run should also specify the same sequencing kit.",
"level": 60,
"level_name": "ERROR",
"name": "audit_inconsistent_sequencing_kit",
"path": "/auxiliary-sets/IGVFDS8878JVPZ/"
},
{
"category": "inconsistent sequencing kit",
"detail": "Auxiliary set [IGVFDS8878JVPZ](/auxiliary-sets/IGVFDS8878JVPZ/) has a sequence file [IGVFFI8600ACAI](/sequence-files/IGVFFI8600ACAI/) sequenced on a `sequencing_platform` [EFO_0008637](/platform-terms/EFO_0008637/) that is inconsistent with its `sequencing_kit` NovaSeq X Series 25B Reagent Kit. Sequence files should specify a sequencing kit which is consistent with their sequencing platform. Sequence files in the same sequencing run should also specify the same sequencing kit.",
"level": 60,
"level_name": "ERROR",
"name": "audit_inconsistent_sequencing_kit",
"path": "/auxiliary-sets/IGVFDS8878JVPZ/"
},
{
"category": "inconsistent sequencing kit",
"detail": "Auxiliary set [IGVFDS8878JVPZ](/auxiliary-sets/IGVFDS8878JVPZ/) has a sequence file [IGVFFI1686TDZN](/sequence-files/IGVFFI1686TDZN/) sequenced on a `sequencing_platform` [EFO_0008637](/platform-terms/EFO_0008637/) that is inconsistent with its `sequencing_kit` NovaSeq X Series 25B Reagent Kit. Sequence files should specify a sequencing kit which is consistent with their sequencing platform. Sequence files in the same sequencing run should also specify the same sequencing kit.",
"level": 60,
"level_name": "ERROR",
"name": "audit_inconsistent_sequencing_kit",
"path": "/auxiliary-sets/IGVFDS8878JVPZ/"
}
],
"INTERNAL_ACTION": [
{
"category": "missing sequence specification",
"detail": "Auxiliary set [IGVFDS8878JVPZ](/auxiliary-sets/IGVFDS8878JVPZ/) has sequence file(s): [IGVFFI1394EWKL](/sequence-files/IGVFFI1394EWKL/), [IGVFFI7023BMQV](/sequence-files/IGVFFI7023BMQV/), [IGVFFI8600ACAI](/sequence-files/IGVFFI8600ACAI/), [IGVFFI1686TDZN](/sequence-files/IGVFFI1686TDZN/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/auxiliary-sets/IGVFDS8878JVPZ/"
}
]
},
"award": {
"@id": "/awards/HG011996/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/95d84f03-8ce0-489c-a755-f33ced139c5d/",
"title": "Gary Hon"
},
"title": "Multiscale functional characterization of genomic variation in human developmental disorders"
},
"control_for": [],
"creation_timestamp": "2024-08-16T20:05:26.254912+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO1756PPKO/",
"accession": "IGVFDO1756PPKO",
"aliases": [
"igvf:donor_of_WTC11"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"file_set_type": "cell hashing barcode sequencing",
"files": [
{
"@id": "/sequence-files/IGVFFI1394EWKL/",
"accession": "IGVFFI1394EWKL",
"aliases": [
"gary-hon:LW437C_6_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-11-13T19:59:43.218187+00:00",
"file_format": "fastq",
"file_size": 4372711432,
"href": "/sequence-files/IGVFFI1394EWKL/@@download/IGVFFI1394EWKL.fastq.gz",
"s3_uri": "s3://igvf-files/2024/11/13/e2be2455-a92d-4451-8295-33bc88f4097b/IGVFFI1394EWKL.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0008637/",
"status": "preview",
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/HTO//LW437C_S31_L006_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7023BMQV/",
"accession": "IGVFFI7023BMQV",
"aliases": [
"gary-hon:LW437C_6_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-11-13T19:58:30.099122+00:00",
"file_format": "fastq",
"file_size": 405595974,
"href": "/sequence-files/IGVFFI7023BMQV/@@download/IGVFFI7023BMQV.fastq.gz",
"s3_uri": "s3://igvf-files/2024/11/13/130ad985-4242-4f28-b9ae-49e5eca3f95e/IGVFFI7023BMQV.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0008637/",
"status": "preview",
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/HTO//LW437C_S31_L006_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8600ACAI/",
"accession": "IGVFFI8600ACAI",
"aliases": [
"gary-hon:LW437C_6_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-11-13T19:58:56.532513+00:00",
"file_format": "fastq",
"file_size": 4087790329,
"href": "/sequence-files/IGVFFI8600ACAI/@@download/IGVFFI8600ACAI.fastq.gz",
"s3_uri": "s3://igvf-files/2024/11/13/eed21e2a-1de4-4f74-a8d1-57012745d378/IGVFFI8600ACAI.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0008637/",
"status": "preview",
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/HTO//LW437C_S31_L006_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1686TDZN/",
"accession": "IGVFFI1686TDZN",
"aliases": [
"gary-hon:LW437C_6_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-11-13T19:58:43.424799+00:00",
"file_format": "fastq",
"file_size": 394970311,
"href": "/sequence-files/IGVFFI1686TDZN/@@download/IGVFFI1686TDZN.fastq.gz",
"s3_uri": "s3://igvf-files/2024/11/13/5568818c-15b1-4d21-8998-00e3da58166f/IGVFFI1686TDZN.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0008637/",
"status": "preview",
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/HTO//LW437C_S31_L006_I2_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS4252LFRU/"
],
"lab": {
"@id": "/labs/gary-hon/",
"title": "Gary Hon, UT"
},
"measurement_sets": [
{
"@id": "/measurement-sets/IGVFDS9614JGLX/",
"accession": "IGVFDS9614JGLX",
"aliases": [
"gary-hon:differentited_WTC11_CM_Pilot2_Mini_PB9_LW437"
],
"preferred_assay_title": "Perturb-seq",
"status": "preview"
}
],
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM8072YOLO/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8072YOLO",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot2_Mini_PB9_LW437"
],
"classifications": [
"differentiated cell specimen"
],
"construct_library_sets": [
"/construct-library-sets/IGVFDS0346MQBH/"
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:WTC11"
],
"status": "released",
"summary": "GM25256 (WTC-11)",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW437), male, Homo sapiens modified with CRISPRi Sp-dCas9, transfected with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
},
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "preview",
"submitted_by": {
"@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
"title": "Mpathi Nzima"
},
"submitted_files_timestamp": "2024-11-13T19:58:30.099122+00:00",
"summary": "cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"uuid": "16df5318-ccd1-4b61-b7c5-3a6fd191d308"
}