HG012053

Ian Whaling

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach

charles-gersbach

Charles Gersbach, Duke

Gregory Crawford

Tim Reddy

audit_missing_seqspec

audit_biosample_age

CRISPR FACS screen

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Alejandro Barrera

gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in CD2

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CRISPR interference FACS screen targeting CD2: alignments

IGVFDS8876GCAP

Sequencing Results (Illumina)

Attribution

IGVFDS8876GCAP

Sequencing Results (Illumina)

Samples

Donors

Related Measurement Sets

File Sets Using This Auxiliary Set as an Input

Attribution