{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS8866USWP/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS8866USWP",
"aliases": [
"charles-gersbach:cd8-tcells-auxiliary-set-crispri-cd2-high-bin-rep2"
],
"audit": {
"NOT_COMPLIANT": [
{
"category": "missing sequence specification",
"detail": "Auxiliary set [IGVFDS8866USWP](/auxiliary-sets/IGVFDS8866USWP/) has sequence file(s): [IGVFFI8750UNIJ](/sequence-files/IGVFFI8750UNIJ/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_missing_seqspec",
"path": "/auxiliary-sets/IGVFDS8866USWP/"
}
],
"WARNING": [
{
"category": "missing age",
"detail": "Primary cell [IGVFSM7658WDNM](/primary-cells/IGVFSM7658WDNM/) is missing `upper_bound_age`, `lower_bound_age`, and `age_units`. Tissues, primary cells, and whole organisms are expected to specify a lower bound age, upper bound age, and age units.",
"level": 40,
"level_name": "WARNING",
"name": "audit_biosample_age",
"path": "/primary-cells/IGVFSM7658WDNM/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/5d329c0e-3ea9-49bf-9bb9-844a53a882c0/",
"title": "Charles Gersbach"
},
"title": "High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes"
},
"control_for": [],
"creation_timestamp": "2024-02-05T22:39:21.545140+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO4080HFHD/",
"accession": "IGVFDO4080HFHD",
"aliases": [
"charles-gersbach:cd8-tcells-donor-15"
],
"sex": "unspecified",
"status": "released",
"taxa": "Homo sapiens"
}
],
"file_set_type": "gRNA sequencing",
"files": [
{
"@id": "/sequence-files/IGVFFI8750UNIJ/",
"accession": "IGVFFI8750UNIJ",
"aliases": [
"charles-gersbach:cd8-tcells-measurement-set-crispri-cd2-high-bin-rep2-R1"
],
"content_type": "reads",
"creation_timestamp": "2023-11-08T20:27:57.053687+00:00",
"file_format": "fastq",
"file_size": 29355991,
"href": "/sequence-files/IGVFFI8750UNIJ/@@download/IGVFFI8750UNIJ.fastq.gz",
"s3_uri": "s3://igvf-files/2023/11/08/d0bfa0a9-78cd-442b-bc93-8b9859ae7b63/IGVFFI8750UNIJ.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0004205/",
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM8-High-R2_S14_L001_R1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS5498STRV/"
],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"measurement_sets": [
{
"@id": "/measurement-sets/IGVFDS7661MIEF/",
"accession": "IGVFDS7661MIEF",
"aliases": [
"charles-gersbach:cd8-tcells-measurement-set-crispri-cd2-high-bin-rep2"
],
"preferred_assay_title": "CRISPR FACS screen"
}
],
"publications": [
{
"@id": "/publications/6cc80a53-4ca1-4bd4-9942-f0f335b7ec20/",
"publication_identifiers": [
"PMID:37945901",
"doi:10.1038/s41588-023-01554-0",
"PMCID:PMC10703699"
]
}
],
"release_timestamp": "2024-11-18T21:09:03.500970+00:00",
"samples": [
{
"@id": "/primary-cells/IGVFSM7658WDNM/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7658WDNM",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispri-cd2-high-bin-rep2"
],
"classifications": [
"primary cell"
],
"construct_library_sets": [
"/construct-library-sets/IGVFDS8345ZMZW/"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"schema_version": "11",
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_files_timestamp": "2023-11-08T20:27:57.053687+00:00",
"summary": "gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in CD2",
"uuid": "d20758db-244b-4da5-be34-e62958e24983"
}