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IGVFDS8794YVNF

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep1_S6
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep2_S61
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep1_S12
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep2_S67
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep1_S18
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep2_S73
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep1_S24
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep2_S79

Sequencing Results

8 items
fastq
reads
1
Illumina HiSeq 2500
AGTTGCTT:TCTC
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TACTTAGC:TGCG
42 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
AGTTGCTT:TGCG
37 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TTCGCTGA:TCTC
40 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TCTGGCGA:TCTC
13 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TACTTAGC:TCTC
25 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TTCGCTGA:TGCG
32 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TCTGGCGA:TGCG
36 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep1_S6
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep2_S61
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep1_S12
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep2_S67
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep1_S18
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep2_S73
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep1_S24
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep2_S79
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford