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IGVFDS8726ORVQ

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This object is in preview status. Metadata is subject to change until this object is released.
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
gary-hon:CM_Pilot_2_Mini_sgRNA_Lenti_LW272

Sequencing Results (Illumina)

4 items
fastq
reads
R2
2
AAF5CKNM5
1
668 MB
Gary Hon, UT
validated
fastq
reads
I1
2
AAF5CKNM5
1
205 MB
Gary Hon, UT
validated
fastq
reads
I2
2
AAF5CKNM5
1
170 MB
Gary Hon, UT
validated
fastq
reads
R1
2
AAF5CKNM5
1
590 MB
Gary Hon, UT
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW272), female, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_H9
female
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:CM_Pilot_2_Mini_Lenti_LW272
Gary Hon, UT

File Sets Using This Auxiliary Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix, unfiltered global differential expression, unfiltered local differential expression
  • gary-hon:H9_shen_construct_sgRNA_Lenti_PrimaryAnalysis
Gary Hon, UT

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT