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IGVFDS8029DSPH

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep1
Publications

Sequencing Results (Illumina)

8 items
fastq
reads
R2
1
HMCCWDSX3
1
4.5 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
1
HMCCWDSX3
2
2.5 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
1
HMCCWDSX3
3
2.5 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
1
HMCCWDSX3
1
2.5 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
1
HMCCWDSX3
2
4.4 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
1
HMCCWDSX3
4
4.4 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
1
HMCCWDSX3
3
4.4 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
1
HMCCWDSX3
4
2.5 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-6
unspecified
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
  • charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep1
Charles Gersbach, Duke

File Sets Using This Auxiliary Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
interference Perturb-seq: filtered feature barcode matrix
  • charles-gersbach:cd8-tcells-intermediate-analysis-set-perturb-seq-crispri
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke