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IGVFDS7947IZNE

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin6hrs-FF3

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
480 B
validated

Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

6 items
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinC-PCR1_S75
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinC-PCR2_S81
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinC-PCR3_S171
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinC-PCR4_S177
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinA-PCR1_S73
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinA-PCR2_S79
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinA-PCR3_S169
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinA-PCR4_S175
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinB-PCR1_S74
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinB-PCR2_S80
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinB-PCR3_S170
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinB-PCR4_S176
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinD-PCR1_S76
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinD-PCR2_S82
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinD-PCR3_S172
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinD-PCR4_S178
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinF-PCR1_S78
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinF-PCR2_S84
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinF-PCR3_S174
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinF-PCR4_S180
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinE-PCR1_S77
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinE-PCR2_S83
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinE-PCR3_S173
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF3-BinE-PCR4_S179
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford