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IGVFDS7828ZYHS

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Aliases
charles-gersbach:jurkats-auxiliary-set-crispra-vp64x2-il2ra-high-bin-rep3
Publications

Sequencing Results (Illumina)

1 item
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
R1
3
000000000-JFKPJ
1
26 MB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:jurkat-tcells-donor-6
unspecified
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
  • charles-gersbach:jurkats-measurement-set-crispra-vp64x2-il2ra-high-bin-rep3
Charles Gersbach, Duke

File Sets Using This Auxiliary Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR activation FACS screen targeting IL2RA: alignments
  • charles-gersbach:jurkats-intermediate-analysis-crispra-vp64x2-il2ra
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke