{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS7773OUJT/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS7773OUJT",
"aliases": [
"jesse-engreitz:auxiliary_NOS3_CRISPRi_FF_R1_FFrep1_binC_PCR2_S195"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing sequence specification",
"detail": "Auxiliary set [IGVFDS7773OUJT](/auxiliary-sets/IGVFDS7773OUJT/) has sequence file(s): [IGVFFI0592LKKK](/sequence-files/IGVFFI0592LKKK/), [IGVFFI0747RCCZ](/sequence-files/IGVFFI0747RCCZ/), [IGVFFI4681ZUAG](/sequence-files/IGVFFI4681ZUAG/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/auxiliary-sets/IGVFDS7773OUJT/"
},
{
"category": "NTR term ID",
"detail": "Sample term for [NTR_0000753](/sample-terms/NTR_0000753/) has been newly requested. Ontology terms with a newly requested term ID are expected to have it updated following its addition to the appropriate ontology database.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_ntr_term_id",
"path": "/sample-terms/NTR_0000753/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"control_for": [],
"creation_timestamp": "2024-04-15T23:17:06.289331+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"accession": "IGVFDO3459DFTW",
"aliases": [
"igvf:donor_of_teloHAEC"
],
"sex": "female",
"status": "released",
"taxa": "Homo sapiens"
}
],
"file_set_type": "gRNA sequencing",
"files": [
{
"@id": "/sequence-files/IGVFFI0592LKKK/",
"accession": "IGVFFI0592LKKK",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-16T21:43:11.156644+00:00",
"file_format": "fastq",
"file_size": 7202854,
"href": "/sequence-files/IGVFFI0592LKKK/@@download/IGVFFI0592LKKK.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/16/02f49ab9-db9a-44d5-8823-83609f516376/IGVFFI0592LKKK.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0004205/",
"status": "released",
"submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep1-BinC-PCRrep2_S195_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI0747RCCZ/",
"accession": "IGVFFI0747RCCZ",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-16T21:43:12.665081+00:00",
"file_format": "fastq",
"file_size": 7083212,
"href": "/sequence-files/IGVFFI0747RCCZ/@@download/IGVFFI0747RCCZ.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/16/0d1c87cd-48cf-4066-8f5c-7a70fc2ffe39/IGVFFI0747RCCZ.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0004205/",
"status": "released",
"submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep1-BinC-PCRrep2_S195_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI4681ZUAG/",
"accession": "IGVFFI4681ZUAG",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-16T21:43:14.254562+00:00",
"file_format": "fastq",
"file_size": 20078385,
"href": "/sequence-files/IGVFFI4681ZUAG/@@download/IGVFFI4681ZUAG.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/16/f273d9ca-2a99-4930-bcff-6c70fe9d1e68/IGVFFI4681ZUAG.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0004205/",
"status": "released",
"submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep1-BinC-PCRrep2_S195_R1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS7917YHKA/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"measurement_sets": [
{
"@id": "/measurement-sets/IGVFDS5321JEBO/",
"accession": "IGVFDS5321JEBO",
"aliases": [
"jesse-engreitz:measurement_NOS3_CRISPRi_FF_R1_FFrep1_binC_PCR2_S195"
],
"preferred_assay_title": "CRISPR FlowFISH screen",
"status": "released"
}
],
"release_timestamp": "2024-06-22T00:27:11.627134+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM3948PYPE/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3948PYPE",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1_binC_PCR2_S195"
],
"classifications": [
"cell line"
],
"construct_library_sets": [
"/construct-library-sets/IGVFDS8953DFJK/"
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:TeloHAEC"
],
"status": "released",
"summary": "TeloHAEC",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-04-16T21:43:11.156644+00:00",
"summary": "gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3",
"uuid": "29e4d16b-ca56-49b6-91b4-9242c9ae2d88"
}