Home

IGVFDS7634SPOA

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR1_Rep1_S4
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR1_Rep2_S59
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR2_Rep1_S10
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR2_Rep2_S65
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR3_Rep1_S16
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR3_Rep2_S71
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR4_Rep1_S22
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR4_Rep2_S77

Sequencing Results

8 items
fastq
reads
1
AAGGATGT:TCTC
14 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ATTATGTT:TGCG
40 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AAGGATGT:TGCG
40 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ATTATGTT:TCTC
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TGCTCGAC:TCTC
18 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TGCTCGAC:TGCG
34 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GGTCCAGA:TCTC
29 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GGTCCAGA:TGCG
29 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR1_Rep1_S4
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR1_Rep2_S59
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR2_Rep1_S10
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR2_Rep2_S65
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR3_Rep1_S16
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR3_Rep2_S71
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR4_Rep1_S22
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR4_Rep2_S77
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford