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IGVFDS7080OXFZ

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR4_S107
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR1_S5
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR2_S11
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR3_S101

Sequencing Results

4 items
fastq
reads
1
AGCAATTC:CTCT
21 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GTATAACA:CTCT
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GTATAACA:ATTG
39 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AGCAATTC:ATTG
45 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR4_S107
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR1_S5
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR2_S11
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR3_S101
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford