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IGVFDS6296UIVP

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR1_Rep1_S30
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR1_Rep2_S85
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR2_Rep1_S36
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR2_Rep2_S91
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR3_Rep1_S42
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR3_Rep2_S97
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR4_Rep1_S48
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR4_Rep2_S103

Sequencing Results

8 items
fastq
reads
1
TTGAATAG:TGCG
33 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TTGAATAG:TCTC
22 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TAATGAAC:TGCG
46 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TAATGAAC:TCTC
28 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ATAGCGTC:TGCG
47 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AGGTTATC:TCTC
19 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AGGTTATC:TGCG
52 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ATAGCGTC:TCTC
14 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR1_Rep1_S30
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR1_Rep2_S85
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR2_Rep1_S36
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR2_Rep2_S91
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR3_Rep1_S42
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR3_Rep2_S97
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR4_Rep1_S48
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR4_Rep2_S103
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford