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IGVFDS6020CDHF

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin6hrs-FF1

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
478 B
validated

Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

6 items
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR1-Rep1_S29
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR2-Rep1_S35
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR3-Rep1_S83
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR4-Rep1_S89
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR1-Rep1_S28
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR2-Rep1_S34
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR3-Rep1_S82
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR4-Rep1_S88
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinA-PCR1-Rep1_S25
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinA-PCR2-Rep1_S31
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinA-PCR3-Rep1_S79
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinA-PCR4-Rep1_S85
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR1-Rep1_S30
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR2-Rep1_S36
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR3-Rep1_S84
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR4-Rep1_S90
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinB-PCR1-Rep1_S26
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinB-PCR2-Rep1_S32
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinB-PCR3-Rep1_S80
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinB-PCR4-Rep1_S86
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinC-PCR1-Rep1_S27
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinC-PCR2-Rep1_S33
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinC-PCR3-Rep1_S81
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinC-PCR4-Rep1_S87
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford