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IGVFDS5951VSHM

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin0hrs-FF1

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
456 B
validated

Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

6 items
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep1_S1
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep2_S56
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep1_S7
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep2_S62
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep1_S13
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep2_S68
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep1_S19
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep2_S74
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR1_Rep1_S3
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR1_Rep2_S58
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR2_Rep1_S9
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR2_Rep2_S64
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR3_Rep1_S15
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR3_Rep2_S70
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR4_Rep1_S21
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR4_Rep2_S76
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep1_S6
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep2_S61
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep1_S12
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep2_S67
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep1_S18
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep2_S73
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep1_S24
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep2_S79
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep1_S2
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep2_S57
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep1_S8
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep2_S63
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep2_S69
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep1_S20
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep2_S75
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep1_S5
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep2_S60
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep1_S11
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep2_S66
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep1_S17
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep2_S72
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep1_S23
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep2_S78
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR1_Rep1_S4
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR1_Rep2_S59
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR2_Rep1_S10
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR2_Rep2_S65
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR3_Rep1_S16
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR3_Rep2_S71
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR4_Rep1_S22
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR4_Rep2_S77
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford