{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS5841QQSW/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS5841QQSW",
"aliases": [
"gary-hon:CM_Pilot_2_Mini_sgRNA_PB_LW273"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing sequencing kit",
"detail": "Auxiliary set [IGVFDS5841QQSW](/auxiliary-sets/IGVFDS5841QQSW/) has sequence file(s) [IGVFFI0200HHMT](/sequence-files/IGVFFI0200HHMT/), [IGVFFI2329KMIP](/sequence-files/IGVFFI2329KMIP/), [IGVFFI6130CCFL](/sequence-files/IGVFFI6130CCFL/), [IGVFFI2017KAHP](/sequence-files/IGVFFI2017KAHP/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/auxiliary-sets/IGVFDS5841QQSW/"
},
{
"category": "missing sequence specification",
"detail": "Auxiliary set [IGVFDS5841QQSW](/auxiliary-sets/IGVFDS5841QQSW/) has sequence file(s): [IGVFFI0200HHMT](/sequence-files/IGVFFI0200HHMT/), [IGVFFI2329KMIP](/sequence-files/IGVFFI2329KMIP/), [IGVFFI6130CCFL](/sequence-files/IGVFFI6130CCFL/), [IGVFFI2017KAHP](/sequence-files/IGVFFI2017KAHP/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/auxiliary-sets/IGVFDS5841QQSW/"
}
]
},
"award": {
"@id": "/awards/HG011996/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/95d84f03-8ce0-489c-a755-f33ced139c5d/",
"title": "Gary Hon"
},
"title": "Multiscale functional characterization of genomic variation in human developmental disorders"
},
"control_for": [],
"creation_timestamp": "2024-04-25T21:44:13.854448+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO2718IDDQ/",
"accession": "IGVFDO2718IDDQ",
"aliases": [
"igvf:donor_of_H9"
],
"sex": "female",
"status": "released",
"taxa": "Homo sapiens"
}
],
"file_set_type": "gRNA sequencing",
"files": [
{
"@id": "/sequence-files/IGVFFI0200HHMT/",
"accession": "IGVFFI0200HHMT",
"aliases": [
"gary-hon:LW273B_1_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-25T22:58:34.105645+00:00",
"file_format": "fastq",
"file_size": 644016319,
"href": "/sequence-files/IGVFFI0200HHMT/@@download/IGVFFI0200HHMT.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/25/96eaa7e2-3a7f-4cd9-9385-7a32a0b7770d/IGVFFI0200HHMT.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0010963/",
"status": "preview",
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-09-11-McDermott-NextSeq.10X/2023-09-11/outs/fastq_path/AACYY52M5//LW273B_S13_L001_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2329KMIP/",
"accession": "IGVFFI2329KMIP",
"aliases": [
"gary-hon:LW273B_1_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-25T22:58:28.231224+00:00",
"file_format": "fastq",
"file_size": 216986468,
"href": "/sequence-files/IGVFFI2329KMIP/@@download/IGVFFI2329KMIP.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/25/e9e1ef7e-d245-4ee8-aef2-e8151ec8cabd/IGVFFI2329KMIP.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0010963/",
"status": "preview",
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-09-11-McDermott-NextSeq.10X/2023-09-11/outs/fastq_path/AACYY52M5//LW273B_S13_L001_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI6130CCFL/",
"accession": "IGVFFI6130CCFL",
"aliases": [
"gary-hon:LW273B_1_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-25T22:58:44.506148+00:00",
"file_format": "fastq",
"file_size": 1099983191,
"href": "/sequence-files/IGVFFI6130CCFL/@@download/IGVFFI6130CCFL.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/25/6a762ae1-ca46-41a3-b721-046004655114/IGVFFI6130CCFL.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0010963/",
"status": "preview",
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-09-11-McDermott-NextSeq.10X/2023-09-11/outs/fastq_path/AACYY52M5//LW273B_S13_L001_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2017KAHP/",
"accession": "IGVFFI2017KAHP",
"aliases": [
"gary-hon:LW273B_1_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-25T22:58:22.216651+00:00",
"file_format": "fastq",
"file_size": 243940675,
"href": "/sequence-files/IGVFFI2017KAHP/@@download/IGVFFI2017KAHP.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/25/dfd3c0f3-0489-48c0-bd7d-e98014af7be5/IGVFFI2017KAHP.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0010963/",
"status": "preview",
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-09-11-McDermott-NextSeq.10X/2023-09-11/outs/fastq_path/AACYY52M5//LW273B_S13_L001_I1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS4762GRIW/"
],
"lab": {
"@id": "/labs/gary-hon/",
"title": "Gary Hon, UT"
},
"measurement_sets": [
{
"@id": "/measurement-sets/IGVFDS1050IJPU/",
"accession": "IGVFDS1050IJPU",
"aliases": [
"gary-hon:CM_Pilot_2_Mini_PB_LW273"
],
"preferred_assay_title": "Perturb-seq",
"status": "preview"
}
],
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM1063UQVF/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1063UQVF",
"aliases": [
"gary-hon:differentiated_H9_shen_construct_sgRNA_PB_LW273"
],
"classifications": [
"differentiated cell specimen"
],
"construct_library_sets": [
"/construct-library-sets/IGVFDS9629YZLO/"
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0003045/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:H9"
],
"status": "released",
"summary": "H9",
"term_name": "H9"
}
],
"status": "preview",
"summary": "H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW273), female, Homo sapiens modified with CRISPRi Sp-dCas9, transfected with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
},
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "preview",
"submitted_by": {
"@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
"title": "Mpathi Nzima"
},
"submitted_files_timestamp": "2024-04-25T22:58:22.216651+00:00",
"summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"uuid": "c2b641e3-78dd-4c38-9010-5d058204e51b"
}