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IGVFDS5620SSUW

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR1-Rep1_S3
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR2-Rep1_S9
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR3-Rep1_S57
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR4-Rep1_S63

Sequencing Results

4 items
fastq
reads
1
GCACATCT:GCCA
24 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ACCAACTG:GCCA
28 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ACCAACTG:TTGA
30 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GCACATCT:TTGA
26 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR1-Rep1_S3
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR2-Rep1_S9
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR3-Rep1_S57
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR4-Rep1_S63
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford