{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS5581ABLR/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS5581ABLR",
"aliases": [
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep1_S5",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep2_S60",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep1_S11",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep2_S66",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep1_S17",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep2_S72",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep1_S23",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep2_S78"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing sequencing kit",
"detail": "Auxiliary set [IGVFDS5581ABLR](/auxiliary-sets/IGVFDS5581ABLR/) has sequence file(s) [IGVFFI9575IHNR](/sequence-files/IGVFFI9575IHNR/), [IGVFFI7367BUVJ](/sequence-files/IGVFFI7367BUVJ/), [IGVFFI1014ZQSZ](/sequence-files/IGVFFI1014ZQSZ/), [IGVFFI8734SFUZ](/sequence-files/IGVFFI8734SFUZ/), [IGVFFI4836TTVK](/sequence-files/IGVFFI4836TTVK/), [IGVFFI1666IWMV](/sequence-files/IGVFFI1666IWMV/), [IGVFFI0132JLWC](/sequence-files/IGVFFI0132JLWC/), [IGVFFI5386XIKZ](/sequence-files/IGVFFI5386XIKZ/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/auxiliary-sets/IGVFDS5581ABLR/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
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"donors": [
{
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"aliases": [
"igvf:donor_of_HCT116"
],
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"status": "released",
"taxa": "Homo sapiens"
}
],
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{
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],
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"upload_status": "validated"
},
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],
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"upload_status": "validated"
},
{
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],
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"upload_status": "validated"
},
{
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],
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1014ZQSZ/",
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],
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"href": "/sequence-files/IGVFFI1014ZQSZ/@@download/IGVFFI1014ZQSZ.fastq.gz",
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8734SFUZ/",
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],
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI4836TTVK/",
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],
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},
{
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],
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},
{
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],
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"upload_status": "validated"
},
{
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],
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},
{
"@id": "/configuration-files/IGVFFI9587XVMG/",
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},
{
"@id": "/configuration-files/IGVFFI5630OODH/",
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},
{
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},
{
"@id": "/configuration-files/IGVFFI8014JOBB/",
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],
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},
{
"@id": "/configuration-files/IGVFFI6337ALBZ/",
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},
{
"@id": "/configuration-files/IGVFFI3637ZSNH/",
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],
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}
],
"input_for": [
"/analysis-sets/IGVFDS4544YEQM/"
],
"lab": {
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"title": "Jesse Engreitz, Stanford"
},
"measurement_sets": [
{
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"accession": "IGVFDS6323AUPJ",
"aliases": [
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"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep2_S60",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep1_S11",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep2_S66",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep1_S17",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep2_S72",
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"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep2_S78"
],
"preferred_assay_title": "CRISPR FlowFISH screen",
"status": "released"
}
],
"release_timestamp": "2024-07-10T20:19:44.130145+00:00",
"samples": [
{
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"Biosample",
"Sample",
"Item"
],
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"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep2_S60",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep1_S11",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep2_S66",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep1_S17",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep2_S72",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep1_S23",
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],
"classifications": [
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],
"construct_library_sets": [
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],
"modifications": [
{
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},
{
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}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:HCT116"
],
"status": "released",
"summary": "HCT116",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-06-01T01:58:13.987116+00:00",
"summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"uuid": "83cb3e5f-a4fa-457d-b228-2739486a78ab"
}