{
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],
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],
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{
"category": "missing sequence specification",
"detail": "Auxiliary set [IGVFDS4935NKQB](/auxiliary-sets/IGVFDS4935NKQB/) has sequence file(s): [IGVFFI4402LQGT](/sequence-files/IGVFFI4402LQGT/), [IGVFFI4297RVGB](/sequence-files/IGVFFI4297RVGB/), [IGVFFI8799UTTR](/sequence-files/IGVFFI8799UTTR/), [IGVFFI8421YAUU](/sequence-files/IGVFFI8421YAUU/) which do not have any `seqspecs`. Sequence files in a file set associated with single cell data are expected to link to a sequence specification file.",
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"path": "/auxiliary-sets/IGVFDS4935NKQB/"
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"title": "Gary Hon"
},
"title": "Multiscale functional characterization of genomic variation in human developmental disorders"
},
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"accession": "IGVFDS0346MQBH",
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{
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"accession": "IGVFFI9879UTKX",
"content_type": "guide RNA sequences",
"status": "in progress",
"summary": "guide RNA sequences"
}
],
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"summary": "guide (sgRNA) library targeting accessible genome regions in many genomic loci"
}
],
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"creation_timestamp": "2024-08-16T20:05:10.103080+00:00",
"data_use_limitation_summaries": [
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],
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{
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"accession": "IGVFDO1756PPKO",
"aliases": [
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],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"file_set_type": "gRNA sequencing",
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{
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"creation_timestamp": "2025-01-14T21:36:31.585241+00:00",
"file_format": "fastq",
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"md5sum": "e1386abbc42cd8814e6b7380e9ae7dc3",
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},
{
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],
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8799UTTR/",
"accession": "IGVFFI8799UTTR",
"aliases": [
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],
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"controlled_access": false,
"creation_timestamp": "2025-01-14T21:36:44.237750+00:00",
"file_format": "fastq",
"file_size": 3486084867,
"href": "/sequence-files/IGVFFI8799UTTR/@@download/IGVFFI8799UTTR.fastq.gz",
"md5sum": "a30c482916d2f412177b3a9e42c0c1ec",
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"status": "preview",
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8421YAUU/",
"accession": "IGVFFI8421YAUU",
"aliases": [
"gary-hon:LW429B_6_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2025-01-14T21:37:22.830786+00:00",
"file_format": "fastq",
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"href": "/sequence-files/IGVFFI8421YAUU/@@download/IGVFFI8421YAUU.fastq.gz",
"md5sum": "28215d1587a714b65a03bd5be146ad99",
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"sequencing_platform": "/platform-terms/EFO_0022841/",
"status": "preview",
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW429B_S15_L006_R2_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS5498SDRJ/"
],
"lab": {
"@id": "/labs/gary-hon/",
"title": "Gary Hon, UT"
},
"measurement_sets": [
{
"@id": "/measurement-sets/IGVFDS3695BOGD/",
"accession": "IGVFDS3695BOGD",
"aliases": [
"gary-hon:differentited_WTC11_CM_Pilot2_Mini_LPv4_LW429"
],
"preferred_assay_title": "Perturb-seq",
"status": "preview"
}
],
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM7766HOYF/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7766HOYF",
"aliases": [
"nikhil-munshi:differentited_WTC11_CM_Pilot2_Mini_LPv4_LW429"
],
"cellular_sub_pool": "subpool_LW429",
"classifications": [
"differentiated cell specimen"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview"
}
],
"sample_terms": [
{
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"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:WTC11"
],
"status": "released",
"summary": "GM25256 (WTC-11)",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW429) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
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"status": "released",
"term_name": "cardiac muscle cell"
},
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "preview",
"submitted_by": {
"@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
"title": "Mpathi Nzima"
},
"submitted_files_timestamp": "2025-01-14T21:36:18.972633+00:00",
"summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"uuid": "1b009bbc-c4db-47dd-ba78-ab3ecf7f5372"
}