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IGVFDS4894APIT

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep1_S114
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep2_S169
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep1_S120
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep2_S175
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep1_S126
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep2_S181
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep1_S132
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep2_S187

Sequencing Results

8 items
fastq
reads
1
GGTCCAGA:GCCA
18 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GGTCCAGA:TTGA
28 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ATTATGTT:GCCA
25 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TGCTCGAC:GCCA
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ATTATGTT:TTGA
27 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AAGGATGT:TTGA
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TGCTCGAC:TTGA
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AAGGATGT:GCCA
24 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep1_S114
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep2_S169
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep1_S120
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep2_S175
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep1_S126
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep2_S181
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep1_S132
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep2_S187
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford