IGVFDS4701YCEL

released
{
    "@context": "/terms/",
    "@id": "/auxiliary-sets/IGVFDS4701YCEL/",
    "@type": [
        "AuxiliarySet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS4701YCEL",
    "aliases": [
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep1_S2",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep2_S57",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep1_S8",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep2_S63",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep2_S69",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep1_S20",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep2_S75"
    ],
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Auxiliary set [IGVFDS4701YCEL](/auxiliary-sets/IGVFDS4701YCEL/) has sequence file(s) [IGVFFI6952RHQM](/sequence-files/IGVFFI6952RHQM/), [IGVFFI1122HKWU](/sequence-files/IGVFFI1122HKWU/), [IGVFFI1697DZHB](/sequence-files/IGVFFI1697DZHB/), [IGVFFI8224NGZQ](/sequence-files/IGVFFI8224NGZQ/), [IGVFFI7565VCLO](/sequence-files/IGVFFI7565VCLO/), [IGVFFI3742WWYT](/sequence-files/IGVFFI3742WWYT/), [IGVFFI7880EKFD](/sequence-files/IGVFFI7880EKFD/), [IGVFFI1267BFAZ](/sequence-files/IGVFFI1267BFAZ/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/auxiliary-sets/IGVFDS4701YCEL/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_for": [],
    "creation_timestamp": "2024-05-21T17:12:23.160528+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO0384OZRW/",
            "accession": "IGVFDO0384OZRW",
            "aliases": [
                "igvf:donor_of_HCT116"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "file_set_type": "gRNA sequencing",
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            ],
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            "controlled_access": false,
            "creation_timestamp": "2024-06-01T01:58:04.312138+00:00",
            "file_format": "fastq",
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            "sequencing_platform": "/platform-terms/EFO_0008565/",
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            "upload_status": "validated"
        },
        {
            "@id": "/configuration-files/IGVFFI1477HXVA/",
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                "jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep1_S2"
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI1122HKWU/",
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        },
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        },
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            "@id": "/sequence-files/IGVFFI8224NGZQ/",
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        },
        {
            "@id": "/configuration-files/IGVFFI7757NDSP/",
            "accession": "IGVFFI7757NDSP",
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                "jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep1_S8"
            ],
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            "upload_status": "validated"
        },
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            "@id": "/configuration-files/IGVFFI4681EQRI/",
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            "status": "released",
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI7565VCLO/",
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            ],
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI3742WWYT/",
            "accession": "IGVFFI3742WWYT",
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                "jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14"
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        },
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            "@id": "/sequence-files/IGVFFI7880EKFD/",
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        },
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            "@id": "/sequence-files/IGVFFI1267BFAZ/",
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        },
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            "@id": "/configuration-files/IGVFFI5950IUVE/",
            "accession": "IGVFFI5950IUVE",
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        },
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            "@id": "/configuration-files/IGVFFI0280XBBS/",
            "accession": "IGVFFI0280XBBS",
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        },
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            "@id": "/configuration-files/IGVFFI7382XUPK/",
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        },
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            "@id": "/configuration-files/IGVFFI1890CWFB/",
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        },
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            "@id": "/configuration-files/IGVFFI6222XYKL/",
            "accession": "IGVFFI6222XYKL",
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                "jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep1_S20"
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        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS4544YEQM/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "measurement_sets": [
        {
            "@id": "/measurement-sets/IGVFDS5560HMQA/",
            "accession": "IGVFDS5560HMQA",
            "aliases": [
                "jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep1_S2",
                "jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep2_S57",
                "jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep1_S8",
                "jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep2_S63",
                "jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14",
                "jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep2_S69",
                "jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep1_S20",
                "jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep2_S75"
            ],
            "preferred_assay_title": "CRISPR FlowFISH screen",
            "status": "released"
        }
    ],
    "release_timestamp": "2024-07-10T20:19:21.541767+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM3954RGXF/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM3954RGXF",
            "aliases": [
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep1_S2",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep2_S57",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep1_S8",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep2_S63",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep2_S69",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep1_S20",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep2_S75"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS7857SYCK/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
                    "modality": "interference",
                    "status": "released"
                },
                {
                    "@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
                    "modality": "degradation",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002824/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:HCT116"
                    ],
                    "status": "released",
                    "summary": "HCT116",
                    "term_name": "HCT116"
                }
            ],
            "status": "released",
            "summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "12",
    "status": "released",
    "submitted_by": {
        "@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
        "title": "Chongyon Park"
    },
    "submitted_files_timestamp": "2024-06-01T01:58:04.312138+00:00",
    "summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
    "uuid": "156deefa-faaf-4730-8478-43866c648284"
}