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IGVFDS4597CMXX

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 3 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin0hrs-FF2

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
481 B
validated

Samples

6 items
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting accessible genome regions in many genomic loci

Related Measurement Sets

5 items
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR1-Rep1_S18
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR2-Rep1_S24
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR3-Rep1_S72
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR4-Rep1_S78
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR1-Rep1_S16
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR2-Rep1_S22
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR3-Rep1_S70
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR4-Rep1_S76
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinC-PCR1-Rep1_S15
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinC-PCR2-Rep1_S21
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinC-PCR3-Rep1_S69
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinC-PCR4-Rep1_S75
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinE-PCR1-Rep1_S17
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinE-PCR2-Rep1_S23
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinE-PCR3-Rep1_S71
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinE-PCR4-Rep1_S77
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR1-Rep1_S14
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR2-Rep1_S20
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR3-Rep1_S68
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR4-Rep1_S74
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford