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IGVFDS4580MCDT

revoked
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR1_Rep1_S137
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR1_Rep2_S192
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR2_Rep1_S143
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR2_Rep2_S198
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR3_Rep1_S149
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR3_Rep2_S204
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR4_Rep1_S155
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR4_Rep2_S210
Revoke Detail
This auxiliary set has been revoked as its files have been moved to the measurement set: /measurement-sets/IGVFDS6319CCJO/
Attribution
Jesse Engreitz, Stanford

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting accessible genome regions in many genomic loci

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR1_Rep1_S137
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR1_Rep2_S192
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR2_Rep1_S143
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR2_Rep2_S198
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR3_Rep1_S149
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR3_Rep2_S204
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR4_Rep1_S155
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR4_Rep2_S210
Jesse Engreitz, Stanford