{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS4549GERY/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS4549GERY",
"aliases": [
"jesse-engreitz:auxiliary_NOS3_CRISPRi_FF_R1_FFrep2_binC_PCR2_S203"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "NTR term ID",
"detail": "Sample term for [NTR_0000753](/sample-terms/NTR_0000753/) has been newly requested. Ontology terms with a newly requested term ID are expected to have it updated following its addition to the appropriate ontology database.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_ntr_term_id",
"path": "/sample-terms/NTR_0000753/"
}
],
"NOT_COMPLIANT": [
{
"category": "missing sequence specification",
"detail": "Auxiliary set [IGVFDS4549GERY](/auxiliary-sets/IGVFDS4549GERY/) has sequence file(s): [IGVFFI7293HSCQ](/sequence-files/IGVFFI7293HSCQ/), [IGVFFI3108NCGS](/sequence-files/IGVFFI3108NCGS/), [IGVFFI9338PAKH](/sequence-files/IGVFFI9338PAKH/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_missing_seqspec",
"path": "/auxiliary-sets/IGVFDS4549GERY/"
}
]
},
"award": {
"@id": "/awards/HG011986/",
"component": "mapping",
"contact_pi": {
"@id": "/users/33c6eb70-e143-4f77-9661-4b3905737daa/",
"title": "Jason Buenrostro"
},
"title": "A foundational resource of functional elements, TF footprints and gene regulatory interactions"
},
"control_for": [],
"creation_timestamp": "2024-04-15T22:47:16.587084+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"accession": "IGVFDO3459DFTW",
"aliases": [
"igvf:donor_of_teloHAEC"
],
"sex": "female",
"status": "released",
"taxa": "Homo sapiens"
}
],
"file_set_type": "gRNA sequencing",
"files": [
{
"@id": "/sequence-files/IGVFFI7293HSCQ/",
"accession": "IGVFFI7293HSCQ",
"content_type": "reads",
"creation_timestamp": "2024-04-16T21:43:48.981544+00:00",
"file_format": "fastq",
"file_size": 9661996,
"href": "/sequence-files/IGVFFI7293HSCQ/@@download/IGVFFI7293HSCQ.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/16/ee62f270-8f59-4871-b845-1a9682330c2d/IGVFFI7293HSCQ.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0004205/",
"submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep2-BinC-PCRrep2_S203_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3108NCGS/",
"accession": "IGVFFI3108NCGS",
"content_type": "reads",
"creation_timestamp": "2024-04-16T21:43:47.559018+00:00",
"file_format": "fastq",
"file_size": 9874725,
"href": "/sequence-files/IGVFFI3108NCGS/@@download/IGVFFI3108NCGS.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/16/44d6c15f-ebff-46a4-afde-d0b8c46e5551/IGVFFI3108NCGS.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0004205/",
"submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep2-BinC-PCRrep2_S203_I1_001.fastq.",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI9338PAKH/",
"accession": "IGVFFI9338PAKH",
"content_type": "reads",
"creation_timestamp": "2024-04-16T21:43:50.511011+00:00",
"file_format": "fastq",
"file_size": 27792627,
"href": "/sequence-files/IGVFFI9338PAKH/@@download/IGVFFI9338PAKH.fastq.gz",
"s3_uri": "s3://igvf-files/2024/04/16/c05e5ce2-8c55-4275-8d46-2a97d1890780/IGVFFI9338PAKH.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0004205/",
"submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep2-BinC-PCRrep2_S203_R1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_file_set_for": [
"/analysis-sets/IGVFDS7917YHKA/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"measurement_sets": [
{
"@id": "/measurement-sets/IGVFDS3743BFON/",
"accession": "IGVFDS3743BFON",
"aliases": [
"jesse-engreitz:measurement_NOS3_CRISPRi_FF_R1_FFrep2_binC_PCR2_S203"
],
"preferred_assay_title": "CRISPR FlowFISH screen"
}
],
"release_timestamp": "2024-06-22T00:27:14.848059+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM4987QNQG/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4987QNQG",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2_binC_PCR2_S203"
],
"classifications": [
"cell line"
],
"construct_library_sets": [
"/construct-library-sets/IGVFDS8953DFJK/"
],
"modifications": [
"/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/"
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:TeloHAEC"
],
"status": "released",
"summary": "TeloHAEC",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "11",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-04-16T21:43:47.559018+00:00",
"summary": "gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3",
"uuid": "31c427d4-5d04-45c6-931c-c8518c40ac46"
}