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IGVFDS4549GERY

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
Aliases
jesse-engreitz:auxiliary_NOS3_CRISPRi_FF_R1_FFrep2_binC_PCR2_S203

Sequencing Results (Illumina)

3 items
fastq
reads
I1
1
9.9 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
9.7 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
28 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_teloHAEC
female
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
  • jesse-engreitz:measurement_NOS3_CRISPRi_FF_R1_FFrep2_binC_PCR2_S203
Jesse Engreitz, Stanford

Attribution

Award
HG011986
Principal Investigator(s)
Contact P.I.
Jason Buenrostro
Lab
Jesse Engreitz, Stanford