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IGVFDS4521OAKZ

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin6hrs-FF1

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
481 B
validated

Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

6 items
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinA-PCR1_S49
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinA-PCR2_S55
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinA-PCR3_S145
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinA-PCR4_S151
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinB-PCR1_S50
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinB-PCR2_S56
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinB-PCR3_S146
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinB-PCR4_S152
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinF-PCR1_S54
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinF-PCR2_S60
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinF-PCR3_S150
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinF-PCR4_S156
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR1_S51
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR2_S57
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR3_S147
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR4_S153
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR1_S52
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR2_S58
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR3_S148
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR4_S154
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinE-PCR4_S155
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinE-PCR1_S53
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinE-PCR2_S59
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinE-PCR3_S149
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford