{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS4432QTTQ/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS4432QTTQ",
"aliases": [
"jesse-engreitz:auxiliary_NOS3_CRISPRi_FF_R2_FFrep2_binB_PCR2_S218"
],
"audit": {
"ERROR": [
{
"category": "missing measurement set",
"detail": "Auxiliary set [IGVFDS4432QTTQ](/auxiliary-sets/IGVFDS4432QTTQ/) is not associated with any `measurement_sets`. Auxiliary sets are expected to be associated with a measurement set(s).",
"level": 60,
"level_name": "ERROR",
"name": "audit_missing_measurement_sets",
"path": "/auxiliary-sets/IGVFDS4432QTTQ/"
}
],
"INTERNAL_ACTION": [
{
"category": "NTR term ID",
"detail": "Sample term for [NTR_0000753](/sample-terms/NTR_0000753/) has been newly requested. Ontology terms with a newly requested term ID are expected to have it updated following its addition to the appropriate ontology database.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_ntr_term_id",
"path": "/sample-terms/NTR_0000753/"
}
],
"NOT_COMPLIANT": [
{
"category": "missing files",
"detail": "Auxiliary set [IGVFDS4432QTTQ](/auxiliary-sets/IGVFDS4432QTTQ/) has no `files`. File sets are expected to have files.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_no_files",
"path": "/auxiliary-sets/IGVFDS4432QTTQ/"
}
],
"WARNING": [
{
"category": "missing nucleic acid delivery",
"detail": "In vitro system [IGVFSM8559QOCC](/in-vitro-systems/IGVFSM8559QOCC/) has `construct_library_sets` but is missing `nucleic_acid_delivery`. Samples linked to construct library sets are expected to specify nucleic acid delivery method.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_nucleic_acid_delivery",
"path": "/in-vitro-systems/IGVFSM8559QOCC/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS8953DFJK/",
"accession": "IGVFDS8953DFJK",
"file_set_type": "guide library",
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI5833LNWN/",
"accession": "IGVFFI5833LNWN",
"content_type": "guide RNA sequences",
"status": "released",
"summary": "GRCh38 guide RNA sequences"
}
],
"status": "released",
"summary": "guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3"
}
],
"control_for": [],
"creation_timestamp": "2024-04-15T23:17:29.112012+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"accession": "IGVFDO3459DFTW",
"aliases": [
"igvf:donor_of_teloHAEC"
],
"sex": "female",
"status": "released",
"taxa": "Homo sapiens"
}
],
"file_set_type": "gRNA sequencing",
"files": [],
"input_for": [
"/analysis-sets/IGVFDS7917YHKA/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"measurement_sets": [],
"release_timestamp": "2024-06-22T00:26:51.802995+00:00",
"revoke_detail": "This auxiliary set has been revoked as its files have been moved to the measurement set: /measurement-sets/IGVFDS9444AXIW/",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM8559QOCC/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8559QOCC",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2_binB_PCR2_S218"
],
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:TeloHAEC"
],
"status": "released",
"summary": "TeloHAEC",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "revoked",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"summary": "gRNA sequencing",
"uuid": "8c671350-d873-4754-8871-11f72d23b60e"
}