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IGVFDS4215TFWE

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
Aliases
charles-gersbach:cd8-tcells-auxiliary-set-crispr-ko-tfome-high-bin-rep2
Publications

Sequencing Results (Illumina)

1 item
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
R1
2
AACJJ7LM5
1
803 MB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-24
unspecified
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
  • charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-high-bin-rep2
Charles Gersbach, Duke

File Sets Using This Auxiliary Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR knockout FACS screen targeting IL7R: alignments
  • charles-gersbach:cd8-tcells-intermediate-analysis-crispr-ko-tfome
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke