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IGVFDS3945FAEL

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep3
Publications

Sequencing Results (Illumina)

8 items
Read Names
fastq
reads
R1
3
HMCCWDSX3
1
1.3 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
3
HMCCWDSX3
1
2.5 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
3
HMCCWDSX3
3
1.3 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
3
HMCCWDSX3
4
1.3 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
3
HMCCWDSX3
3
2.5 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
3
HMCCWDSX3
2
1.3 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
3
HMCCWDSX3
2
2.5 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
3
HMCCWDSX3
4
2.5 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-8
unspecified
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in 24 genes

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
  • charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep3
Charles Gersbach, Duke

File Sets Using This Auxiliary Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
activation Perturb-seq: differential gene expression quantifications, filtered feature barcode matrix
  • charles-gersbach:cd8-tcells-analysis-set-perturb-seq-crispra
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke