IGVFDS3919NBFE

{
    "@context": "/terms/",
    "@id": "/auxiliary-sets/IGVFDS3919NBFE/",
    "@type": [
        "AuxiliarySet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS3919NBFE",
    "aliases": [
        "gary-hon:differentited_H9_CM_Pilot2_Mini_PB9_sgRNA_LW433"
    ],
    "audit": {
        "ERROR": [
            {
                "category": "inconsistent sequencing kit",
                "detail": "Auxiliary set [IGVFDS3919NBFE](/auxiliary-sets/IGVFDS3919NBFE/) has a sequence file [IGVFFI5800QJKD](/sequence-files/IGVFFI5800QJKD/) sequenced on a `sequencing_platform` [EFO_0008637](/platform-terms/EFO_0008637/) that is inconsistent with its `sequencing_kit` NovaSeq X Series 25B Reagent Kit. Sequence files should specify a sequencing kit which is consistent with their sequencing platform. Sequence files in the same sequencing run should also specify the same sequencing kit.",
                "level": 60,
                "level_name": "ERROR",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/auxiliary-sets/IGVFDS3919NBFE/"
            },
            {
                "category": "inconsistent sequencing kit",
                "detail": "Auxiliary set [IGVFDS3919NBFE](/auxiliary-sets/IGVFDS3919NBFE/) has a sequence file [IGVFFI7530AEJH](/sequence-files/IGVFFI7530AEJH/) sequenced on a `sequencing_platform` [EFO_0008637](/platform-terms/EFO_0008637/) that is inconsistent with its `sequencing_kit` NovaSeq X Series 25B Reagent Kit. Sequence files should specify a sequencing kit which is consistent with their sequencing platform. Sequence files in the same sequencing run should also specify the same sequencing kit.",
                "level": 60,
                "level_name": "ERROR",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/auxiliary-sets/IGVFDS3919NBFE/"
            },
            {
                "category": "inconsistent sequencing kit",
                "detail": "Auxiliary set [IGVFDS3919NBFE](/auxiliary-sets/IGVFDS3919NBFE/) has a sequence file [IGVFFI5201BBTZ](/sequence-files/IGVFFI5201BBTZ/) sequenced on a `sequencing_platform` [EFO_0008637](/platform-terms/EFO_0008637/) that is inconsistent with its `sequencing_kit` NovaSeq X Series 25B Reagent Kit. Sequence files should specify a sequencing kit which is consistent with their sequencing platform. Sequence files in the same sequencing run should also specify the same sequencing kit.",
                "level": 60,
                "level_name": "ERROR",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/auxiliary-sets/IGVFDS3919NBFE/"
            },
            {
                "category": "inconsistent sequencing kit",
                "detail": "Auxiliary set [IGVFDS3919NBFE](/auxiliary-sets/IGVFDS3919NBFE/) has a sequence file [IGVFFI5561SSLS](/sequence-files/IGVFFI5561SSLS/) sequenced on a `sequencing_platform` [EFO_0008637](/platform-terms/EFO_0008637/) that is inconsistent with its `sequencing_kit` NovaSeq X Series 25B Reagent Kit. Sequence files should specify a sequencing kit which is consistent with their sequencing platform. Sequence files in the same sequencing run should also specify the same sequencing kit.",
                "level": 60,
                "level_name": "ERROR",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/auxiliary-sets/IGVFDS3919NBFE/"
            }
        ],
        "INTERNAL_ACTION": [
            {
                "category": "missing sequence specification",
                "detail": "Auxiliary set [IGVFDS3919NBFE](/auxiliary-sets/IGVFDS3919NBFE/) has sequence file(s): [IGVFFI5800QJKD](/sequence-files/IGVFFI5800QJKD/), [IGVFFI7530AEJH](/sequence-files/IGVFFI7530AEJH/), [IGVFFI5201BBTZ](/sequence-files/IGVFFI5201BBTZ/), [IGVFFI5561SSLS](/sequence-files/IGVFFI5561SSLS/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/auxiliary-sets/IGVFDS3919NBFE/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011996/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/95d84f03-8ce0-489c-a755-f33ced139c5d/",
            "title": "Gary Hon"
        },
        "title": "Multiscale functional characterization of genomic variation in human developmental disorders"
    },
    "control_for": [],
    "creation_timestamp": "2024-08-16T20:05:18.061338+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO2718IDDQ/",
            "accession": "IGVFDO2718IDDQ",
            "aliases": [
                "igvf:donor_of_H9"
            ],
            "sex": "female",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "file_set_type": "gRNA sequencing",
    "files": [
        {
            "@id": "/sequence-files/IGVFFI5800QJKD/",
            "accession": "IGVFFI5800QJKD",
            "aliases": [
                "gary-hon:LW433B_6_I2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-13T19:49:18.186879+00:00",
            "file_format": "fastq",
            "file_size": 797103799,
            "href": "/sequence-files/IGVFFI5800QJKD/@@download/IGVFFI5800QJKD.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/13/372f8289-8edd-4c04-9861-7b20a8335a22/IGVFFI5800QJKD.fastq.gz",
            "sequencing_platform": "/platform-terms/EFO_0008637/",
            "status": "preview",
            "submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW433B_S7_L006_I2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI7530AEJH/",
            "accession": "IGVFFI7530AEJH",
            "aliases": [
                "gary-hon:LW433B_6_I1"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-13T19:49:01.558844+00:00",
            "file_format": "fastq",
            "file_size": 794657701,
            "href": "/sequence-files/IGVFFI7530AEJH/@@download/IGVFFI7530AEJH.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/13/9024bd70-8f2c-4074-b99a-a26922a1e74d/IGVFFI7530AEJH.fastq.gz",
            "sequencing_platform": "/platform-terms/EFO_0008637/",
            "status": "preview",
            "submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW433B_S7_L006_I1_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI5201BBTZ/",
            "accession": "IGVFFI5201BBTZ",
            "aliases": [
                "gary-hon:LW433B_6_R1"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-13T19:49:34.046966+00:00",
            "file_format": "fastq",
            "file_size": 6564230005,
            "href": "/sequence-files/IGVFFI5201BBTZ/@@download/IGVFFI5201BBTZ.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/13/25b1a2c8-a413-4d67-bd5c-755851623da1/IGVFFI5201BBTZ.fastq.gz",
            "sequencing_platform": "/platform-terms/EFO_0008637/",
            "status": "preview",
            "submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW433B_S7_L006_R1_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI5561SSLS/",
            "accession": "IGVFFI5561SSLS",
            "aliases": [
                "gary-hon:LW433B_6_R2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-13T19:50:39.527530+00:00",
            "file_format": "fastq",
            "file_size": 6516742074,
            "href": "/sequence-files/IGVFFI5561SSLS/@@download/IGVFFI5561SSLS.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/13/c0e76443-77b1-4301-b221-4ca1021427aa/IGVFFI5561SSLS.fastq.gz",
            "sequencing_platform": "/platform-terms/EFO_0008637/",
            "status": "preview",
            "submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW433B_S7_L006_R2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS2266YDVM/"
    ],
    "lab": {
        "@id": "/labs/gary-hon/",
        "title": "Gary Hon, UT"
    },
    "measurement_sets": [
        {
            "@id": "/measurement-sets/IGVFDS8751LXWJ/",
            "accession": "IGVFDS8751LXWJ",
            "aliases": [
                "gary-hon:differentited_H9_CM_Pilot2_Mini_PB9_LW433"
            ],
            "preferred_assay_title": "Perturb-seq",
            "status": "preview"
        }
    ],
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM1513DHGW/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM1513DHGW",
            "aliases": [
                "nikhil-munshi:differentited_H9_CM_Pilot2_Mini_PB9_LW433"
            ],
            "classifications": [
                "differentiated cell specimen"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS0346MQBH/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
                    "modality": "interference",
                    "status": "preview"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0003045/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:H9"
                    ],
                    "status": "released",
                    "summary": "H9",
                    "term_name": "H9"
                }
            ],
            "status": "preview",
            "summary": "H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW433), female, Homo sapiens modified with CRISPRi Sp-dCas9, transfected with a guide library",
            "targeted_sample_term": {
                "@id": "/sample-terms/CL_0000746/",
                "status": "released",
                "term_name": "cardiac muscle cell"
            },
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "12",
    "status": "preview",
    "submitted_by": {
        "@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
        "title": "Mpathi Nzima"
    },
    "submitted_files_timestamp": "2024-11-13T19:49:01.558844+00:00",
    "summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
    "uuid": "b86be77d-fa7f-42fa-b964-13bdd186c531"
}