{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS3914IPWH/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS3914IPWH",
"aliases": [
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep1_S112",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep2_S167",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep1_S118",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep2_S173",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep1_S124",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep1_S130",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing sequencing kit",
"detail": "Auxiliary set [IGVFDS3914IPWH](/auxiliary-sets/IGVFDS3914IPWH/) has sequence file(s) [IGVFFI4264KJXK](/sequence-files/IGVFFI4264KJXK/), [IGVFFI3058GAYK](/sequence-files/IGVFFI3058GAYK/), [IGVFFI8232UPNC](/sequence-files/IGVFFI8232UPNC/), [IGVFFI7993WRUA](/sequence-files/IGVFFI7993WRUA/), [IGVFFI8689TPHH](/sequence-files/IGVFFI8689TPHH/), [IGVFFI1193HDXG](/sequence-files/IGVFFI1193HDXG/), [IGVFFI1593YXMS](/sequence-files/IGVFFI1593YXMS/), [IGVFFI6277LDPG](/sequence-files/IGVFFI6277LDPG/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/auxiliary-sets/IGVFDS3914IPWH/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"control_for": [],
"creation_timestamp": "2024-05-21T17:39:07.102437+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO0384OZRW/",
"accession": "IGVFDO0384OZRW",
"aliases": [
"igvf:donor_of_HCT116"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
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{
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"accession": "IGVFFI4264KJXK",
"aliases": [
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],
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"file_format": "fastq",
"file_size": 18100081,
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"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI4823SDRE/",
"accession": "IGVFFI4823SDRE",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep1_S112"
],
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"file_format": "yaml",
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"href": "/configuration-files/IGVFFI4823SDRE/@@download/IGVFFI4823SDRE.yaml.gz",
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3058GAYK/",
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"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179"
],
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"creation_timestamp": "2024-06-01T02:05:19.001380+00:00",
"file_format": "fastq",
"file_size": 26578251,
"href": "/sequence-files/IGVFFI3058GAYK/@@download/IGVFFI3058GAYK.fastq.gz",
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"sequencing_platform": "/platform-terms/EFO_0008565/",
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"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI9689CPCW/",
"accession": "IGVFFI9689CPCW",
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"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185"
],
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"file_format": "yaml",
"file_size": 912,
"href": "/configuration-files/IGVFFI9689CPCW/@@download/IGVFFI9689CPCW.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/e4e6671a-9269-42d8-8e46-ba32e2c7d1a1/IGVFFI9689CPCW.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185.yaml",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8232UPNC/",
"accession": "IGVFFI8232UPNC",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep1_S130"
],
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"controlled_access": false,
"creation_timestamp": "2024-06-01T02:05:37.654908+00:00",
"file_format": "fastq",
"file_size": 18577055,
"href": "/sequence-files/IGVFFI8232UPNC/@@download/IGVFFI8232UPNC.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/69f84bb3-109b-4596-bc1b-256401cef427/IGVFFI8232UPNC.fastq.gz",
"sequencing_platform": "/platform-terms/EFO_0008565/",
"status": "released",
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"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI1475ALYJ/",
"accession": "IGVFFI1475ALYJ",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep1_S124"
],
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"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI1475ALYJ/@@download/IGVFFI1475ALYJ.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/84c02af7-d303-4bae-8fb1-65868b34d378/IGVFFI1475ALYJ.yaml.gz",
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7993WRUA/",
"accession": "IGVFFI7993WRUA",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep2_S167"
],
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"creation_timestamp": "2024-06-01T02:03:58.458025+00:00",
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8689TPHH/",
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],
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"creation_timestamp": "2024-06-01T02:04:38.923088+00:00",
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"href": "/sequence-files/IGVFFI8689TPHH/@@download/IGVFFI8689TPHH.fastq.gz",
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},
{
"@id": "/sequence-files/IGVFFI1193HDXG/",
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],
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1593YXMS/",
"accession": "IGVFFI1593YXMS",
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],
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},
{
"@id": "/sequence-files/IGVFFI6277LDPG/",
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],
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},
{
"@id": "/configuration-files/IGVFFI1900MCOG/",
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],
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},
{
"@id": "/configuration-files/IGVFFI5990DFIF/",
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],
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},
{
"@id": "/configuration-files/IGVFFI8825EPCR/",
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"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep1_S118"
],
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"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI5156TDWH/",
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],
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"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI7482TIZK/",
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],
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"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS4544YEQM/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"measurement_sets": [
{
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"accession": "IGVFDS6517MOFU",
"aliases": [
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"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep2_S167",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep1_S118",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep2_S173",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep1_S124",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep1_S130",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185"
],
"preferred_assay_title": "CRISPR FlowFISH screen",
"status": "released"
}
],
"release_timestamp": "2024-07-10T20:20:54.278106+00:00",
"samples": [
{
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"@type": [
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"Biosample",
"Sample",
"Item"
],
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"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep2_S167",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep1_S118",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep2_S173",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep1_S124",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep1_S130",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185"
],
"classifications": [
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],
"construct_library_sets": [
"/construct-library-sets/IGVFDS7857SYCK/"
],
"modifications": [
{
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},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
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}
],
"sample_terms": [
{
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"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:HCT116"
],
"status": "released",
"summary": "HCT116",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/d35868d1-c45b-49ae-8ff3-a9c0c22551c2/",
"status": "released",
"treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid"
}
]
}
],
"schema_version": "12",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-06-01T02:03:38.334076+00:00",
"summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"uuid": "134a2818-52d3-4762-aa07-8b604a23ea3a"
}