HG012053

Ian Whaling

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach

charles-gersbach

Charles Gersbach, Duke

Gregory Crawford

Tim Reddy

audit_missing_seqspec

CRISPR FACS screen

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Alejandro Barrera

gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

CRISPR activation FACS screen targeting IL2RA: alignments

IGVFDS3792YMVB

Sequencing Results (Illumina)

Attribution

IGVFDS3792YMVB

Sequencing Results (Illumina)

Samples

Donors

Related Measurement Sets

File Sets Using This Auxiliary Set as an Input

Attribution