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IGVFDS3139RYUI

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep1_S27
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep2_S82
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep1_S33
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep2_S88
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep1_S39
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep2_S94
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep1_S45
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep2_S100

Sequencing Results

8 items
fastq
reads
1
CGTTACCA:TGCG
40 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GACAGTAA:TGCG
37 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCCAGCAA:TGCG
41 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCTCGGTC:TGCG
39 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GACAGTAA:TCTC
15 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
CGTTACCA:TCTC
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCTCGGTC:TCTC
22 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCCAGCAA:TCTC
15 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep1_S27
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep2_S82
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep1_S33
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep2_S88
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep1_S39
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep2_S94
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep1_S45
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep2_S100
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford