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IGVFDS2855OFEM

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR1-Rep1_S29
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR2-Rep1_S35
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR3-Rep1_S83
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR4-Rep1_S89

Sequencing Results

4 items
fastq
reads
1
TCGCCTTG:GCCA
23 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TTACGCAC:TTGA
14 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TTACGCAC:GCCA
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCGCCTTG:TTGA
17 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR1-Rep1_S29
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR2-Rep1_S35
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR3-Rep1_S83
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR4-Rep1_S89
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford