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IGVFDS2834VAYS

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep1_S26
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep2_S81
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep1_S32
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep1_S38
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep2_S93
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep1_S44
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99

Sequencing Results

8 items
fastq
reads
1
Illumina HiSeq 2500
ATTCTAGG:TCTC
28 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
CCTTCGCA:TGCG
38 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
AGGATCTA:TCTC
29 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
CAGCAAGG:TGCG
34 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
ATTCTAGG:TGCG
46 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
AGGATCTA:TGCG
52 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
CCTTCGCA:TCTC
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
CAGCAAGG:TCTC
8.9 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep1_S26
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep2_S81
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep1_S32
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep1_S38
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep2_S93
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep1_S44
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford