IGVFDS2805FQAD

released
{
    "@context": "/terms/",
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    "@type": [
        "AuxiliarySet",
        "FileSet",
        "Item"
    ],
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    "aliases": [
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep1_S1",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep2_S56",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep1_S7",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep2_S62",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep1_S13",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep2_S68",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep1_S19",
        "jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep2_S74"
    ],
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Auxiliary set [IGVFDS2805FQAD](/auxiliary-sets/IGVFDS2805FQAD/) has sequence file(s) [IGVFFI8310ZXDQ](/sequence-files/IGVFFI8310ZXDQ/), [IGVFFI9473RAYZ](/sequence-files/IGVFFI9473RAYZ/), [IGVFFI7962MDES](/sequence-files/IGVFFI7962MDES/), [IGVFFI1423QMWB](/sequence-files/IGVFFI1423QMWB/), [IGVFFI7230YSEB](/sequence-files/IGVFFI7230YSEB/), [IGVFFI5243KTWV](/sequence-files/IGVFFI5243KTWV/), [IGVFFI8079GYLE](/sequence-files/IGVFFI8079GYLE/), [IGVFFI5803HMJL](/sequence-files/IGVFFI5803HMJL/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/auxiliary-sets/IGVFDS2805FQAD/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
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        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_for": [],
    "creation_timestamp": "2024-05-21T17:12:21.856032+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO0384OZRW/",
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                "igvf:donor_of_HCT116"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
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            "@id": "/configuration-files/IGVFFI5319VYHW/",
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    "input_for": [
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    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
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                "jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep2_S74"
            ],
            "preferred_assay_title": "CRISPR FlowFISH screen",
            "status": "released"
        }
    ],
    "release_timestamp": "2024-07-10T20:19:14.452384+00:00",
    "samples": [
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            "@id": "/in-vitro-systems/IGVFSM3086DQMC/",
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                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep1_S7",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep2_S62",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep1_S13",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep2_S68",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep1_S19",
                "jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep2_S74"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS7857SYCK/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
                    "modality": "interference",
                    "status": "released"
                },
                {
                    "@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
                    "modality": "degradation",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002824/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:HCT116"
                    ],
                    "status": "released",
                    "summary": "HCT116",
                    "term_name": "HCT116"
                }
            ],
            "status": "released",
            "summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "12",
    "status": "released",
    "submitted_by": {
        "@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
        "title": "Chongyon Park"
    },
    "submitted_files_timestamp": "2024-06-01T01:58:01.097868+00:00",
    "summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
    "uuid": "da116226-ab43-4a55-bd1c-ac9cdf19010c"
}