{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS2606RJRX/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS2606RJRX",
"aliases": [
"charles-gersbach:cd8-tcells-auxiliary-set-crispr-ko-tfome-batf3-over-low-bin-rep1"
],
"audit": {
"ERROR": [
{
"category": "missing measurement set",
"detail": "Auxiliary set [IGVFDS2606RJRX](/auxiliary-sets/IGVFDS2606RJRX/) is not associated with any `measurement_sets`. Auxiliary sets are expected to be associated with a measurement set(s).",
"level": 60,
"level_name": "ERROR",
"name": "audit_missing_measurement_sets",
"path": "/auxiliary-sets/IGVFDS2606RJRX/"
}
],
"NOT_COMPLIANT": [
{
"category": "missing files",
"detail": "Auxiliary set [IGVFDS2606RJRX](/auxiliary-sets/IGVFDS2606RJRX/) has no `files`. File sets are expected to have files.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_no_files",
"path": "/auxiliary-sets/IGVFDS2606RJRX/"
}
],
"WARNING": [
{
"category": "missing age",
"detail": "Primary cell [IGVFSM3895SURE](/primary-cells/IGVFSM3895SURE/) is missing `upper_bound_age`, `lower_bound_age`, and `age_units`. Tissues, primary cells, and whole organisms are expected to specify a lower bound age, upper bound age, and age units.",
"level": 40,
"level_name": "WARNING",
"name": "audit_biosample_age",
"path": "/primary-cells/IGVFSM3895SURE/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/5d329c0e-3ea9-49bf-9bb9-844a53a882c0/",
"title": "Charles Gersbach"
},
"title": "High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS3667PLQB/",
"accession": "IGVFDS3667PLQB",
"file_set_type": "guide library",
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI9735DZKB/",
"accession": "IGVFFI9735DZKB",
"content_type": "guide RNA sequences",
"status": "released",
"summary": "GRCh38 guide RNA sequences"
}
],
"status": "released",
"summary": "guide (sgRNA) library targeting transcription start sites in many genes"
},
{
"@id": "/construct-library-sets/IGVFDS1868WXHB/",
"accession": "IGVFDS1868WXHB",
"file_set_type": "expression vector library",
"status": "released",
"summary": "expression vector library of BATF3"
}
],
"control_for": [],
"creation_timestamp": "2024-02-05T22:39:41.681789+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO8306NDTY/",
"accession": "IGVFDO8306NDTY",
"aliases": [
"charles-gersbach:cd8-tcells-donor-25"
],
"sex": "unspecified",
"status": "released",
"taxa": "Homo sapiens"
}
],
"file_set_type": "gRNA sequencing",
"files": [],
"input_for": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"measurement_sets": [],
"release_timestamp": "2024-11-18T21:09:01.600762+00:00",
"revoke_detail": "This auxiliary set has been revoked as its files have been moved to the measurement set: /measurement-sets/IGVFDS3910ZTSZ/",
"samples": [
{
"@id": "/primary-cells/IGVFSM3895SURE/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3895SURE",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-low-bin-rep1"
],
"classifications": [
"primary cell"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "revoked",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"summary": "gRNA sequencing",
"uuid": "5849bf55-5445-427e-82de-dcfd1509dd31"
}