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INDEXED
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AuxiliarySet
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IGVFDS2399UCGA
IGVFDS2399UCGA
released
Json view
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep2
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI2078FUDW
fastq
reads
R1
2
IGVFFI0543IRBP
Illumina NovaSeq 6000
HMCCWDSX3
1
2.2 GB
Charles Gersbach, Duke
validated
IGVFFI2639BDZX
fastq
reads
R2
2
IGVFFI4432MMHG
Illumina NovaSeq 6000
HMCCWDSX3
4
3.8 GB
Charles Gersbach, Duke
validated
IGVFFI4948ANCU
fastq
reads
R1
2
IGVFFI4432MMHG
Illumina NovaSeq 6000
HMCCWDSX3
4
2.2 GB
Charles Gersbach, Duke
validated
IGVFFI5510BIGH
fastq
reads
R2
2
IGVFFI9274GOYS
Illumina NovaSeq 6000
HMCCWDSX3
3
3.8 GB
Charles Gersbach, Duke
validated
IGVFFI7200EBQJ
fastq
reads
R1
2
IGVFFI9274GOYS
Illumina NovaSeq 6000
HMCCWDSX3
3
2.2 GB
Charles Gersbach, Duke
validated
IGVFFI7327OSWO
fastq
reads
R2
2
IGVFFI0543IRBP
Illumina NovaSeq 6000
HMCCWDSX3
1
3.9 GB
Charles Gersbach, Duke
validated
IGVFFI7473QCSU
fastq
reads
R1
2
IGVFFI2662OZJM
Illumina NovaSeq 6000
HMCCWDSX3
2
2.2 GB
Charles Gersbach, Duke
validated
IGVFFI9451ZFRL
fastq
reads
R2
2
IGVFFI2662OZJM
Illumina NovaSeq 6000
HMCCWDSX3
2
3.8 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM2747RJFY
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO6082RNLT
charles-gersbach:cd8-tcells-donor-7
unspecified
Homo sapiens
Related Measurement Sets
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS1333MBPK
MeasurementSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep2
Charles Gersbach, Duke
File Sets Using This Auxiliary Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS3319RFFN
AnalysisSet
interference Perturb-seq: filtered feature barcode matrix
charles-gersbach:cd8-tcells-intermediate-analysis-set-perturb-seq-crispri
Charles Gersbach, Duke
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke