{
"@context": "/terms/",
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"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS2220HHTN",
"aliases": [
"jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane2_CS1"
],
"audit": {
"ERROR": [
{
"category": "inconsistent sequence specifications",
"detail": "Auxiliary set [IGVFDS2220HHTN](/auxiliary-sets/IGVFDS2220HHTN/) has sequence files: [IGVFFI4137IEHI](/sequence-files/IGVFFI4137IEHI/), [IGVFFI4398ITZJ](/sequence-files/IGVFFI4398ITZJ/), [IGVFFI4961EMLW](/sequence-files/IGVFFI4961EMLW/), [IGVFFI7687RJYL](/sequence-files/IGVFFI7687RJYL/), [IGVFFI7842UOGS](/sequence-files/IGVFFI7842UOGS/), [IGVFFI4732FLWG](/sequence-files/IGVFFI4732FLWG/), [IGVFFI8008YPNQ](/sequence-files/IGVFFI8008YPNQ/), [IGVFFI0849UBQW](/sequence-files/IGVFFI0849UBQW/) which share the same `seqspecs` [IGVFFI0899KKAC](/configuration-files/IGVFFI0899KKAC/), but belong to different sequencing sets. Sequence files in a file set from the same sequencing set are expected to link to the same seqspec file, which should be unique to that sequencing set. A sequencing set is defined by the combination of flowcell ID, lane, and index for non-single cell data, or flowcell ID, lane, index, and sequencing run for single cell data.",
"level": 60,
"level_name": "ERROR",
"name": "audit_inconsistent_seqspec",
"path": "/auxiliary-sets/IGVFDS2220HHTN/"
}
]
},
"award": {
"@id": "/awards/HG011966/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/eb543cda-67b7-493c-84eb-187d0dac13ba/",
"title": "Jay Shendure"
},
"title": "Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems"
},
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}
],
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"summary": "guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
}
],
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"creation_timestamp": "2023-12-08T23:52:06.493785+00:00",
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{
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"aliases": [
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],
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"status": "released",
"taxa": "Homo sapiens"
}
],
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"upload_status": "validated"
},
{
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],
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},
{
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"upload_status": "validated"
},
{
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"creation_timestamp": "2023-12-09T00:51:11.951900+00:00",
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"upload_status": "validated"
},
{
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],
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},
{
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{
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},
{
"@id": "/configuration-files/IGVFFI0899KKAC/",
"accession": "IGVFFI0899KKAC",
"aliases": [
"jay-shendure:seqspec_of_set_IGVFDS2220HHTN"
],
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"creation_timestamp": "2025-04-02T18:30:05.158970+00:00",
"file_format": "yaml",
"file_size": 1735,
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"status": "in progress",
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"upload_status": "validated"
}
],
"input_for": [
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],
"lab": {
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"title": "Jay Shendure, UW"
},
"measurement_sets": [
{
"@id": "/measurement-sets/IGVFDS0272VVTX/",
"accession": "IGVFDS0272VVTX",
"aliases": [
"jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane2_exprs"
],
"preferred_assay_title": "scCRISPR screen",
"status": "released"
}
],
"publications": [
{
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"publication_identifiers": [
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],
"status": "released"
}
],
"release_timestamp": "2024-08-13T20:47:22.455248+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM9913PXTT/",
"@type": [
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"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM9913PXTT",
"aliases": [
"jay-shendure:CRISPRa_VPH_NGN2_WTC11_neurons_10Xlane2"
],
"cellular_sub_pool": "10Xlane2",
"classifications": [
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],
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"modifications": [
{
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"modality": "activation",
"status": "released"
}
],
"sample_terms": [
{
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"@type": [
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"OntologyTerm",
"Item"
],
"aliases": [
"igvf:WTC11"
],
"status": "released",
"summary": "GM25256 (WTC-11)",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "released",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library",
"targeted_sample_term": {
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"status": "released",
"term_name": "neuron"
},
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "released",
"submitted_by": {
"@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
"title": "Beth Martin"
},
"submitted_files_timestamp": "2023-12-09T00:51:03.848506+00:00",
"summary": "gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci",
"uuid": "5cb44b8e-6570-45af-9bf6-4cdf9c01423c"
}