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IGVFDS2059NFJA

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep1_S29
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep2_S84
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep1_S35
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep2_S90
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep1_S41
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep2_S96
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep1_S47
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep2_S102

Sequencing Results

8 items
fastq
reads
1
TTACGCAC:TCTC
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCCTTGGT:TGCG
38 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GAACCTAG:TGCG
49 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GAACCTAG:TCTC
18 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TTACGCAC:TGCG
37 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCGCCTTG:TGCG
37 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCGCCTTG:TCTC
19 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCCTTGGT:TCTC
15 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep1_S29
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep2_S84
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep1_S35
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep2_S90
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep1_S41
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep2_S96
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep1_S47
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep2_S102
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford